Počet záznamů: 1
Physiological and spectroscopical changes of the thermophilic cyanobacterium Synechococcus elongatus under iron stress and recovery culture
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SYSNO ASEP 0542474 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Physiological and spectroscopical changes of the thermophilic cyanobacterium Synechococcus elongatus under iron stress and recovery culture Tvůrce(i) El-Sheekh, Mostafa M. (MBU-M)
Prášil, Ondřej (MBU-M) RID, ORCID
El-Mohsnawy, E. (EG)Číslo článku 72 Zdroj.dok. Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 43, č. 5 (2021)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Iron limitation ; Photosynthesis ; Pigments ; Recovery ; Synechococcus elongatus Obor OECD Microbiology CEP GA18-07822S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000641849900001 EID SCOPUS 85104513918 DOI 10.1007/s11738-021-03242-0 Anotace The iron in cyanobacterial cells contributes to energy conversion via electron transport chains, so its availability directly influences all metabolic pathways. Besides exploring cell adaptations and changes occurring with iron insufficient stress, the present work examines profoundly for the first time the metabolic changes accompanied with iron recovery for the thermophilic cyanobacterium Synechococcus elongatus (Nag., var. thermalis Geitl. Strain Kovrov 1972/8). S. elongatus cells were cultivated on two iron deprivation levels, iron-limited (45 nM Fe) and iron-deficient (4.5 nM Fe). Growth, carotenoids/Chlorophyll-a (Car/Chl-a) ratio, transmitting electron images, pigments fractionation analysis, and cell activity were checked. The obtained results demonstrated an astounding decrease in Chl-a content, an increment in beta-carotene content, leading to rising the Car/Chl-a ratio, a reduction of phycobilins content, a reduction in cell diameters, a decrease of energy transfer, a depletion of the electron transport chain, and a reduction of photosynthesis and respiration processes under inadequate iron condition. Likewise, the photochemical activity of photosystem II (PSII), determined by Fv/Fm ratio and thermoluminescence estimations, demonstrated that PSII was disabled under iron pressure. With iron recovery by 200 nM Fe, cells regained full metabolic activity within 24 h. Despite the fact that photosynthesis and respiration activity exhibited nearly a similar behavior, S. elongatus under iron-deficiency began to recuperate the activity of the photosynthetic apparatus quicker than the respiratory machinery by regaining their pigment content and activity. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://link.springer.com/article/10.1007/s11738-021-03242-0
Počet záznamů: 1