Počet záznamů: 1
A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype
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SYSNO ASEP 0541994 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype Tvůrce(i) Hýžd'alová, M. (CZ)
Procházková, J. (CZ)
Strapáčová, S. (CZ)
Svrzkova, L. (CZ)
Vacek, Ondřej (BFU-R) ORCID
Fedr, Radek (BFU-R) ORCID
Andrysik, Z. (US)
Hrubá, E. (CZ)
Líbalová, Helena (UEM-P) RID
Kléma, J. (CZ)
Topinka, Jan (UEM-P) RID, ORCID
Mašek, J. (CZ)
Souček, Karel (BFU-R) RID, ORCID
Vondráček, Jan (BFU-R) RID, ORCID
Machala, M. (CZ)Celkový počet autorů 15 Číslo článku 128126 Zdroj.dok. Chemosphere. - : Elsevier - ISSN 0045-6535
Roč. 263, JAN 2021 (2021)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova aryl-hydrocarbon receptor ; polycyclic aromatic-hydrocarbons ; tgf-beta ; a549 cells ; cigarette-smoking Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Cell biology Vědní obor RIV – spolupráce Ústav experimentální medicíny - Morfologické obory a cytologie Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 ; UEM-P - RVO:68378041 UT WOS 000595802200223 EID SCOPUS 85091122385 DOI https://doi.org/10.1016/j.chemosphere.2020.128126 Anotace Deciphering the role of the aryl hydrocarbon receptor (AhR) in lung cancer cells may help us to better understand the role of toxic AhR ligands in lung carcinogenesis, including cancer progression. We employed human lung carcinoma A549 cells to investigate their fate after continuous two-week exposure to model AhR agonists, genotoxic benzo[a]pyrene (BaP, 1 mu M) and non-genotoxic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 10 nM). While TCDD increased proliferative rate of A549 cells, exposure to BaP decreased cell proliferation and induced epithelial-to-mesenchymal transition (EMT)-like phenotype, which was associated with enhanced cell migration, invasion, and altered cell morphology. Although TCDD also suppressed expression of E-cadherin and activated some genes linked to EMT, it did not induce the EMT-like phenotype. The results of transcriptomic analysis, and the opposite effects of BaP and TCDD on cell proliferation, indicated that a delay in cell cycle progression, together with a slight increase of senescence (when coupled with AhR activation), favors the induction of EMT-like phenotype. The shift towards EMT-like phenotype observed after simultaneous treatment with TCDD and mitomycin C (an inhibitor of cell proliferation) confirmed the hypothesis. Since BaP decreased cell proliferative rate via induction of p21 expression, we generated the A549 cell model with reduced p21 expression and exposed it to BaP for two weeks. The p21 knockdown suppressed the BaP-mediated EMT-like phenotype in A549 cells, thus confirming that a delayed cell cycle progression, together with p21-dependent induction of senescence-related chemokine CCL2, may contribute to induction of EMT-like cell phenotype in lung cells exposed to genotoxic AhR ligands. (C) 2020 Elsevier Ltd. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0045653520323213?via%3Dihub
Počet záznamů: 1