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Glucocorticoids reset circadian clock in choroid plexus via period genes
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SYSNO ASEP 0541621 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Glucocorticoids reset circadian clock in choroid plexus via period genes Tvůrce(i) Liška, Karolína (FGU-C) ORCID
Sládek, Martin (FGU-C) RID, ORCID, SAI
Čečmanová, Vendula (FGU-C)
Sumová, Alena (FGU-C) RID, ORCIDZdroj.dok. Journal of Endocrinology. - : BioScientifica - ISSN 0022-0795
Roč. 248, č. 2 (2021), s. 155-166Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova choroid plexus ; circadian clock ; glucocorticoids ; dexamethasone ; circadian entrainment ; phase-response curve Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP304/12/G069 GA ČR - Grantová agentura ČR Způsob publikování Open access s časovým embargem (01.02.2022) Institucionální podpora FGU-C - RVO:67985823 UT WOS 000629252300010 EID SCOPUS 85102602029 DOI 10.1530/JOE-20-0526 Anotace The epithelial cells of choroid plexus (CP) in brain ventricles produce cerebrospinal fluid and act as the blood-cerebrospinal fluid barrier. In this study, we confirmed that CP in the 4th ventricle is composed of cellular oscillators that all harbor glucocorticoid receptors and are mutually synchronized to produce a robust clock gene expression rhythm detectable at the tissue level in vivo and in vitro. Animals lacking glucocorticoids (GCs) due to surgical removal of adrenal glands had Per1, Per2, Nr1d1 and Bmal1 clock gene rhythmicity in their CP significantly dampened, whereas subjecting them to daily bouts of synthetic GC analog, dexamethasone (DEX), reinforced those rhythms. We verified these in vivo effects using an in vitro model of organotypic CP explants, depending on the time of its application, DEX significantly increased the amplitude and efficiently reset the phase of the CP clock. The results are the first description of a PRC for a non-neuronal clock in the brain, demonstrating that CP clock shares some properties with the non-neuronal clocks elsewhere in the body. Finally, we found that DEX exhibited multiple synergic effects on the CP clock, including acute activation of Per1 expression and change of PER2 protein turnover rate. The DEX-induced shifts of the CP clock were partially mediated via PKA-ERK1/2 pathway. The results provide the first evidence that the GC rhythm strengthens and entrains the clock in the CP helping thus fine-tune the brain environment according to time of day. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1530/JOE-20-0526
Počet záznamů: 1