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Analysis and characterization of antimicrobial peptides by capillary electromigration methods
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SYSNO ASEP 0538687 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Analysis and characterization of antimicrobial peptides by capillary electromigration methods Tvůrce(i) Kašička, Václav (UOCHB-X) RID, ORCID
Tůmová, Tereza (UOCHB-X) RID
Monincová, Lenka (UOCHB-X)
Čeřovský, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Czech Chemical Society Symposium Series - ISSN 2336-7202
Roč. 18, č. 3 (2020), s. 68Poč.str. 1 s. Forma vydání Tištěná - P Akce Sjezd českých a slovenských chemických společností /72./ Datum konání 06.09.2020 - 09.09.2020 Místo konání Praha Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova antimicrobial peptides ; capillary electrophoresis ; capillary isotachophoresis Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA18-02597S GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 Anotace High-performance capillary electromigration methods, zone electrophoresis (CZE) and isotachophoresis (CITP), have been employed for qualitative and quantitative analysis and physicochemical characterization of polycationic antimicrobial peptides (AMPs) isolated from the venom of two species of Hymenoptera insects. These AMPs are composed of 10–20 amino acids (AAs) with 3 to 7 basic AAs (His, Lys, Arg). Purity degree of synthetic AMPs was quantified by CZE in acidic background electrolytes (BGEs) using capillary coatings with cationic surfactants or polymers suppressing peptide sorption to the capillary wall. Acid-base and electromigration properties of the AMPs were investigated by combination of CZE and CITP. First, the mixed acidity constants, pKa,mix, and the actual ionic mobilities of the AMPs were estimated by nonlinear regression analysis of the pH dependence of their effective mobilities measured in series of BGEs within wide pH range (1.80–12.10), at constant ionic strength (25 mM) and temperature (25 °C). Then, the pKa,mix values were recalculated to the thermodynamic pKas using the DebyeHückel equation for activity coefficients. Effective charge of the AMPs was estimated by CITP. This approach is based on the linear dependence of the CITP zone length of the analyte on its effective charge and injected substance amount. Cationic CITP systems with leading electrolyte (LE) composed of 10 mM ammonium leading ion, 40 mM acetate counterion, pH 4.1, and terminating electrolyte containing 40 mM acetic acid, pH 3.2, provided sharp AMPs zones with good linearity and repeatability of their lengths. Ionic mobilities of AMPs were determined by CZE in the BGE of the same composition as the LE. Using this procedure, the effective charge numbers of the AMPs were found to be in the range 1.65–5.04, i.e. much less than the theoretical charge numbers (2.86–6.99) calculated from the pKa values. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2021
Počet záznamů: 1