Počet záznamů: 1
Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
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SYSNO ASEP 0537933 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies Tvůrce(i) Němčáková, Ivana (FGU-C) RID, ORCID
Blahová, L. (CZ)
Ryšánek, P. (CZ)
Blanquer, Andreu (FGU-C) RID, ORCID
Bačáková, Lucie (FGU-C) RID, ORCID
Zajíčková, L. (CZ)Číslo článku 9467 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 24 (2020)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova amine plasma polymer ; bioactive coating ; polycaprolactone nanofibers ; substrate morphology ; cell adhesion ; cell adhesion Vědní obor RIV BK - Mechanika tekutin Obor OECD Fluids and plasma physics (including surface physics) CEP GA18-12774S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzechNanoLab - 90110 - Vysoké učení technické v Brně Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000602830000001 EID SCOPUS 85097659712 DOI 10.3390/ijms21249467 Anotace Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1422-0067/21/24/9467
Počet záznamů: 1