Počet záznamů: 1
Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
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SYSNO ASEP 0537734 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120 Tvůrce(i) Srivastava, Amit (MBU-M) ORCID
Ballal, A. (IN)
Forchhammer, K. (DE)
Tripathi, A. K. (IN)Číslo článku e3528 Zdroj.dok. Bio-protocol. - : Bio-protocol - ISSN 2331-8325
Roč. 10, č. 4 (2020)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova AntisenseRNA (asRNA) ; Knock-down ; Anabaena PCC 7120 ; Cyanobacteria ; Green fluorescent protein ; psbA1 promoter Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000583091400009 DOI 10.21769/BioProtoc.3528 Anotace Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a bottleneck in the elucidation of its genomic function. Antisense RNA (asRNA) mediated approach renders the study of essential genes possible by suppressing (but not completely eliminating) expression of the target gene, thus allowing them to function to some extent. Recently, we have successfully implemented this approach using the strong endogenous promoter of the psbAl gene (D1 subunit of Photosystem II) introduced into a highcopy replicative plasmid (pAM1956) to suppress the transcript level of the target gene alr0277 (encoding a sigma factor, SigJ/Alr0277) in Anabaena. This protocol represents an efficient and easy procedure to further explore the functional genomics, expanding the scope of basic and applied research in these ecologically important cyanobacteria. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://bio-protocol.org/e3528?sid=f85bba85-5c8b-4cd0-96d2-e2c9090f9d97&sousouid=20210118012731766&ptype=0&px=1&intwzid=3528
Počet záznamů: 1