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12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling
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SYSNO ASEP 0537569 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling Tvůrce(i) Radaszkiewicz, K. A. (CZ)
Beckerov, D. (CZ)
Woloszczukov, L. (CZ)
Radaszkiewicz, T.W. (CZ)
Lesakov, P. (CZ)
Blanářová, O. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Humpolíček, P. (CZ)
Pacherník, J. (CZ)Celkový počet autorů 9 Číslo článku 15922 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova protein-kinase-c ; embryonic stem-cells ; transcription factor gata4 ; hl-60 cells ; differentiation ; cardiomyocytes Vědní obor RIV BO - Biofyzika Obor OECD Biophysics Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000577348000016 DOI 10.1038/s41598-020-73074-4 Anotace 12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6-8 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa http://apps.webofknowledge.com/InboundService.do?customersID=Alerting&mode=FullRecord&IsProductCode=Yes&product=WOS&Init=Yes&Func=Frame&DestFail=http%3A%2F%2Fwww.webofknowledge.com&action=retrieve&SrcApp=Alerting&SrcAuth=Alerting&SID=E67B63irIgusvnWIdyQ&UT=WOS%3A000577348000016
Počet záznamů: 1