Počet záznamů: 1
Age-related differences in the translational landscape of mammalian oocytes
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SYSNO ASEP 0536356 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Age-related differences in the translational landscape of mammalian oocytes Tvůrce(i) del Llano, Edgar (UZFG-Y) ORCID
Mašek, T. (CZ)
Gahurová, Lenka (UZFG-Y) ORCID
Pospíšek, M. (CZ)
Končická, Markéta (UZFG-Y) ORCID
Jindrová, Anna (UZFG-Y) ORCID
Jansová, Denisa (UZFG-Y) ORCID
Iyyappan, Rajan (UZFG-Y) ORCID
Roučová, K. (CZ)
Bruce, A. W. (CZ)
Kubelka, Michal (UZFG-Y) RID, ORCID
Šušor, Andrej (UZFG-Y) RID, ORCIDČíslo článku e13231 Zdroj.dok. Aging Cell. - : Wiley - ISSN 1474-9718
Roč. 19, č. 10 (2020)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova aging ; cytokinesis ; meiosis ; oocyte ; translation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP GA19-13491S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000570994100001 EID SCOPUS 85091109400 DOI 10.1111/acel.13231 Anotace Increasing maternal age in mammals is associated with poorer oocyte quality, involving higher aneuploidy rates and decreased developmental competence. Prior to resumption of meiosis, fully developed mammalian oocytes become transcriptionally silent until the onset of zygotic genome activation. Therefore, meiotic progression and early embryogenesis are driven largely by translational utilization of previously synthesized mRNAs. We report that genome-wide translatome profiling reveals considerable numbers of transcripts that are differentially translated in oocytes obtained from aged compared to young females. Additionally, we show that a number of aberrantly translated mRNAs in oocytes from aged females are associated with cell cycle. Indeed, we demonstrate that four specific maternal age-related transcripts (Sgk1,Castor1,AireandEg5) with differential translation rates encode factors that are associated with the newly forming meiotic spindle. Moreover, we report substantial defects in chromosome alignment and cytokinesis in the oocytes of young females, in which candidate CASTOR1 and SGK1 protein levels or activity are experimentally altered. Our findings indicate that improper translation of specific proteins at the onset of meiosis contributes to increased chromosome segregation problems associated with female ageing. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2021 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1111/acel.13231
Počet záznamů: 1