Počet záznamů: 1  

Age-related differences in the translational landscape of mammalian oocytes

  1. 1.
    SYSNO ASEP0536356
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAge-related differences in the translational landscape of mammalian oocytes
    Tvůrce(i) del Llano, Edgar (UZFG-Y) ORCID
    Mašek, T. (CZ)
    Gahurová, Lenka (UZFG-Y) ORCID
    Pospíšek, M. (CZ)
    Končická, Markéta (UZFG-Y) ORCID
    Jindrová, Anna (UZFG-Y) ORCID
    Jansová, Denisa (UZFG-Y) ORCID
    Iyyappan, Rajan (UZFG-Y) ORCID
    Roučová, K. (CZ)
    Bruce, A. W. (CZ)
    Kubelka, Michal (UZFG-Y) RID, ORCID
    Šušor, Andrej (UZFG-Y) RID, ORCID
    Číslo článkue13231
    Zdroj.dok.Aging Cell. - : Wiley - ISSN 1474-9718
    Roč. 19, č. 10 (2020)
    Poč.str.13 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaaging ; cytokinesis ; meiosis ; oocyte ; translation
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDBiology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
    CEPGA19-13491S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaUZFG-Y - RVO:67985904
    UT WOS000570994100001
    EID SCOPUS85091109400
    DOI10.1111/acel.13231
    AnotaceIncreasing maternal age in mammals is associated with poorer oocyte quality, involving higher aneuploidy rates and decreased developmental competence. Prior to resumption of meiosis, fully developed mammalian oocytes become transcriptionally silent until the onset of zygotic genome activation. Therefore, meiotic progression and early embryogenesis are driven largely by translational utilization of previously synthesized mRNAs. We report that genome-wide translatome profiling reveals considerable numbers of transcripts that are differentially translated in oocytes obtained from aged compared to young females. Additionally, we show that a number of aberrantly translated mRNAs in oocytes from aged females are associated with cell cycle. Indeed, we demonstrate that four specific maternal age-related transcripts (Sgk1,Castor1,AireandEg5) with differential translation rates encode factors that are associated with the newly forming meiotic spindle. Moreover, we report substantial defects in chromosome alignment and cytokinesis in the oocytes of young females, in which candidate CASTOR1 and SGK1 protein levels or activity are experimentally altered. Our findings indicate that improper translation of specific proteins at the onset of meiosis contributes to increased chromosome segregation problems associated with female ageing.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2021
    Elektronická adresahttps://onlinelibrary.wiley.com/doi/full/10.1111/acel.13231
Počet záznamů: 1  

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