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Structural Characterization of the Apoptosis Signal‐regulating Kinase 1 (ASK1) Domain Responsible for Thioredoxin Binding and its Complex
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SYSNO ASEP 0533796 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Structural Characterization of the Apoptosis Signal‐regulating Kinase 1 (ASK1) Domain Responsible for Thioredoxin Binding and its Complex Tvůrce(i) Obšil, T. (CZ)
Pšenáková, K. (CZ)
Hexnerová, Rozálie (UOCHB-X) ORCID, RID
Srb, Pavel (UOCHB-X) RID, ORCID
Obšilová, Veronika (FGU-C) RID, ORCID, SAI
Veverka, Václav (UOCHB-X) RID, ORCIDZdroj.dok. FASEB Journal. - : Wiley - ISSN 0892-6638
Roč. 34, S1 (2020)Poč.str. 1 s. Akce Annual Meeting on Experimental Biology Datum konání 04.04.2020 - 07.04.2020 Místo konání San Diego Země US - Spojené státy americké Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ASK1 ; thioredoxin Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA19-00121S GA ČR - Grantová agentura ČR EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UOCHB-X - RVO:61388963 ; FGU-C - RVO:67985823 UT WOS 000546023103374 DOI 10.1096/fasebj.2020.34.s1.03959 Anotace Apoptosis signal‐regulating kinase 1 (ASK1) is a mitogen‐activated protein kinase kinase kinase 5 implicated in p38 mitogen‐activated protein kinase and c‐Jun N‐terminal kinase signaling cascades. ASK1 responds to a wide range of signaling cues and is dysregulated in cancer, neurodegeneration and cardiovascular diseases. The catalytic activity of ASK1 is tightly controlled through oligomerization and binding of several cofactors, among which thioredoxin stands out as the most important ASK1 inhibitor. Interestingly, only the reduced form of thioredoxin inhibits ASK1 by direct binding to its N‐terminal domain and oxidation‐driven thioredoxin dissociation is the key event in oxidative stress‐mediated ASK1 activation. Despite extensive efforts, the molecular basis of this key cellular regulatory mechanism has remained unknown until now, primarily due to the dynamic properties of the ASK1 N‐terminal domain, which has prevented its structural characterization by X‐ray crystallography. Here, we report the structural characterization of the ASK1 domain responsible for thioredoxin binding and of the ASK1: thioredoxin complex using NMR spectroscopy and chemical cross‐linking, which provides the molecular basis for complex dissociation under oxidative stress conditions. Our data reveal that the N‐terminal domain of ASK1 adopts a fold resembling the thioredoxin structure while retaining substantial conformational plasticity at the thioredoxin‐binding interface. Although oxidative stress induces relatively moderate structural changes in thioredoxin, we show that these changes lead to a substantial conformational rearrangement of the thioredoxin‐binding interface on ASK1 driven by the formation of intramolecular disulfide bridges. Lastly, we demonstrate that the cysteine residue at the position 250 of ASK1 is the key element of this molecular switch. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2021
Počet záznamů: 1