0533336 - BTÚ 2021 RIV US eng J - Článek v odborném periodiku
Huggett, J.F. - Whale, A. S. - De Spiegelaere, W. - Nour, A. A. - Bae, Y.-K. - Benes, V. - Burke, D. - Cleveland, M. - Corbisier, P. - Devonshire, A. S. - Dong, L. - Drandi, D. - Foy, C. A. - Garson, J. A. - He, H.-J. - Hellemans, J. - Kubista, Mikael - Lievens, A. - Makrigiorgos, M. G. - Milavec, M. - Mueller, R. D. - Nolan, T. - O'Sullivan, D. M. - Pfaffl, M. W. - Roediger, S. - Romsos, E. L. - Shipley, G. L. - Taly, V. - Untergasser, A. - Wittwer, C. T. - Bustin, S. A. - Vandesompele, J. … celkem 33 autorů
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020.
Clinical Chemistry. Roč. 66, č. 8 (2020), s. 1012-1029. ISSN 0009-9147. E-ISSN 1530-8561
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: POLYMERASE-CHAIN-REACTION * DNA COPY NUMBER * ABSOLUTE QUANTIFICATION
Obor OECD: Medical laboratory technology (including laboratory samples analysis
Impakt faktor: 8.327, rok: 2020 ; AIS: 2.73, rok: 2020
Způsob publikování: Open access
Web výsledku:
https://academic.oup.com/clinchem/article/66/8/1012/5880117DOI: https://doi.org/10.1093/clinchem/hvaa125

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges, data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.
Trvalý link: http://hdl.handle.net/11104/0311768