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Neonatal Clonazepam Administration Induced Long-Lasting Changes in GABA(A) and GABA(B) Receptors
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SYSNO ASEP 0531146 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Neonatal Clonazepam Administration Induced Long-Lasting Changes in GABA(A) and GABA(B) Receptors Tvůrce(i) Kubová, Hana (FGU-C) RID, ORCID
Bendová, Z. (CZ)
Moravcová, S. (CZ)
Pačesová, D. (CZ)
Rocha, L. (MX)
Mareš, Pavel (FGU-C) RID, ORCIDČíslo článku 3184 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 9 (2020)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova neonatal rat ; clonazepam ; GABA(A)/BZD receptor binding ; GABA(B)receptor binding ; subunit mRNA expression Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA19-11931S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000535581700155 EID SCOPUS 85084276453 DOI 10.3390/ijms21093184 Anotace Benzodiazepines (BZDs) are widely used in patients of all ages. Unlike adults, neonatal animals treated with BZDs exhibit a variety of behavioral deficits later in life, however, the mechanisms underlying these deficits are poorly understood. This study aims to examine whether administration of clonazepam (CZP, 1 mg/kg/day) in 7-11-day-old rats affects Gama aminobutyric acid (GABA)ergic receptors in both the short and long terms. Using RT-PCR and quantitative autoradiography, we examined the expression of the selected GABA(A) receptor subunits (alpha 1, alpha 2, alpha 4, gamma 2, and delta) and the GABA(B) B2 subunit, and GABA(A), benzodiazepine, and GABA(B) receptor binding 48 h, 1 week, and 2 months after treatment discontinuation. Within one week after CZP cessation, the expression of the alpha 2 subunit was upregulated, whereas that of the delta subunit was downregulated in both the hippocampus and cortex. In the hippocampus, the alpha 4 subunit was downregulated after the 2-month interval. Changes in receptor binding were highly dependent on the receptor type, the interval after treatment cessation, and the brain structure. GABA(A) receptor binding was increased in almost all of the brain structures after the 48-h interval. BZD-binding was decreased in many brain structures involved in the neuronal networks associated with emotional behavior, anxiety, and cognitive functions after the 2-month interval. Binding of the GABA(B) receptors changed depending on the interval and brain structure. Overall, the described changes may affect both synaptic development and functioning and may potentially cause behavioral impairment. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1422-0067/21/9/3184
Počet záznamů: 1