SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR
Tvůrce(i)
Novotný, Ivan (UMG-J) Efenberková, Michaela (UMG-J) Čapek, Martin (UMG-J) Horváth, Ondřej (UMG-J)
Zdroj.dok.
Advances in Disease Models. - Praha : OPTIO CZ, 2019 / Bartůněk Petr
- ISBN 978-80-88011-06-4
Rozsah stran
s. 234-240
Poč.str.
7 s.
Poč.výt.
50
Poč.str.knihy
310
Forma vydání
Tištěná - P
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
super-resolution ; STED microscope ; photon
Vědní obor RIV
EB - Genetika a molekulární biologie
Obor OECD
Cell biology
CEP
LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora
UMG-J - RVO:68378050
Anotace
The super-resolution (SR) methods are rapidly developing approaches in light microscopy field. The Stimulated emission depletion (STED) microscopy is SR method based on confocal scanning microscope. During the acquisition scanning, two lasers – excitation and depletion – are co-aligned into one single spot in the sample. For the maximal efficiency of the SR method, the proper co-alignment of used laser beams is crucial. The article explains the STED microscope operator recommended routine for the alignment. Simple experiments demonstrate significantly decreased number of incoming photons in the misaligned system. It naturally leads to the lower resolution during the acquisition and these important aspects are discussed within the article.
