Počet záznamů: 1
Effects of post-mortem and physical degradation on RNA integrity and quality
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SYSNO ASEP 0523050 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název Effects of post-mortem and physical degradation on RNA integrity and quality Tvůrce(i) Šídová, Monika (BTO-N) RID
Tománková, Silvie (BTO-N) RID
Abaffy, Pavel (BTO-N)
Kubista, Mikael (BTO-N) RID
Šindelka, Radek (BTO-N) RIDCelkový počet autorů 5 Zdroj.dok. Biomolecular Detection and Quantification - ISSN 2214-7535
Roč. 5, September 2015 (2015), s. 3-9Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Degradation ; RNA integrity ; RNA quality ; rqi Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 EID SCOPUS 84943627995 DOI 10.1016/j.bdq.2015.08.002 Anotace The precision and reliability of quantitative nucleic acid analysis depends on the quality of the sample analyzed and the integrity of the nucleic acids. The integrity of RNA is currently primarily assessed by the analysis of ribosomal RNA, which is the by far dominant species. The extrapolation of these results to mRNAs and microRNAs, which are structurally quite different, is questionable. Here we show that ribosomal and some nucleolar and mitochondrial RNAs, are highly resistant to naturally occurring post-mortem degradation, while mRNAs, although showing substantial internal variability, are generally much more prone to nucleolytic degradation. In contrast, all types of RNA show the same sensitivity to heat. Using qPCR assays targeting different regions of mRNA molecules, we find no support for 5' or 3' preferentiality upon post-mortem degradation. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2214753515300048?via%3Dihub
Počet záznamů: 1