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MICRONUCLEI LEVELS IN NANOCOMPOSITES PRODUCTION WORKERS: INTERPRETATION OF RESULTS FROM TWO YEARS OF MONITORING
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SYSNO ASEP 0522981 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název MICRONUCLEI LEVELS IN NANOCOMPOSITES PRODUCTION WORKERS: INTERPRETATION OF RESULTS FROM TWO YEARS OF MONITORING Tvůrce(i) Rössnerová, Andrea (UEM-P) RID
Pelcová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Rössner ml., Pavel (UEM-P) RID, ORCID
Elzeinova, Fatima (UEM-P)
Vrbová, Kristýna (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Koštejn, Martin (UCHP-M) RID, SAI, ORCID
Komarc, M. (CZ)
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Lischková, L. (CZ)
Dvořáčková, Š. (CZ)Zdroj.dok. NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. - Ostrava : Tanger Ltd., 2019 - ISBN 978-808729489-5 Rozsah stran s. 554-559 Poč.str. 6 s. Forma vydání Tištěná - P Akce NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./ Datum konání 17.10.2018 - 19.10.2018 Místo konání Brno Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova genotoxicity ; human ; micronucleus assay Vědní obor RIV DN - Vliv životního prostředí na zdraví Obor OECD Public and environmental health Vědní obor RIV – spolupráce Ústav chemických procesů - Vliv životního prostředí na zdraví CEP LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-02079S GA ČR - Grantová agentura ČR Institucionální podpora UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000513131900096 EID SCOPUS 85062991930 Anotace Micronucleus assay, which has been used for almost sixty years, is one of the basic methodological approaches of genetic toxicology. This is also evident from more than 10,500 references found in PubMed database. These studies concern the evaluation of cytotoxicity and genotoxicity of numerous chemicals and in the last decade also nanomaterials (NM) and nanoparticles (NP). Surprisingly, no relevant human studies focused on MN formation following the exposure to NP have been reported.
In our study we analyzed 4x (in September 2016 and 2017, pre-shift and post-shift each year) samples in a group of workers, working for almost 18 years in nanocomposites research, and matched controls. Detail aerosol exposure monitoring of particulate matter (PM) including nano-sized fractions was completed during working shift in a sampling day. The micronucleus assay using Pan-Centromeric Chromosome Paint was applied to recognize, beside the frequency of total micronuclei (MN) in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks), including the centromere positive (CEN+) and centromere negative (CEN-) micronuclei.
The monitoring data showed differences in the risk of exposure to NP related to individual working processes, as well as differences in chemical composition of nano-fraction. Cytogenetic results demonstrated consistently (both years): (i) possible adaptation to long-term exposure of NP (related to total frequency of MN), (ii) short-term (2.5 h) exposure could be a reason for the aberration increase, particularly a chromosomal losses (aneugenic effect).Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2020
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