Comparison of 12 DNA extraction kits for vertebrate samples

0522567 - ÚBO 2021 RIV ES eng J - Článek v odborném periodiku
Martincová, Iva - Aghová, Tatiana
Comparison of 12 DNA extraction kits for vertebrate samples.
Animal Biodiversity And Conservation. Roč. 43, č. 1 (2020), s. 67-77. ISSN 1578-665X. E-ISSN 2014-928X
Institucionální podpora: RVO:68081766
Klíčová slova: DNA concentration * DNA isolation * DNA purity * Price of extraction kit
Obor OECD: Biodiversity conservation
Impakt faktor: 1.000, rok: 2020
Způsob publikování: Open access
https://doi.org/10.32800/abc.2020.43.0067

Papers are published under a. Obtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column–based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick® Genomic DNA Purification Kit (Genomed) and the NucleoSpin® Tissue (Macherey–Nagel). Nevertheless, it is highly recommended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project.
Trvalý link: http://hdl.handle.net/11104/0307038