Počet záznamů: 1
Tracking of Borrelia afzelii Transmission from Infected Ixodes ricinus Nymphs to Mice
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SYSNO ASEP 0520425 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tracking of Borrelia afzelii Transmission from Infected Ixodes ricinus Nymphs to Mice Tvůrce(i) Pospíšilová, Tereza (BC-A) RID
Urbanová, Veronika (BC-A) RID, ORCID
Hes, O. (CZ)
Kopáček, Petr (BC-A) RID, ORCID
Hajdušek, Ondřej (BC-A) RID, ORCID
Šíma, Radek (BC-A) RID, ORCIDCelkový počet autorů 6 Číslo článku e00896-18 Zdroj.dok. Infection and Immunity. - : American Society for Microbiology - ISSN 0019-9567
Roč. 87, č. 6 (2019)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova lyme-disease spirochete ; burgdorferi sensu-stricto ; gene-expression ; north-america ; ticks ; ospc ; time ; kinetics ; protein ; growth ; Borrelia ; Borrelia afzelii ; Ixodes ricinus ; Lyme disease ; tick-borne pathogens ; transmission Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-27393S GA ČR - Grantová agentura ČR GA17-27386S GA ČR - Grantová agentura ČR GA18-01832S GA ČR - Grantová agentura ČR EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000468521100009 EID SCOPUS 85064456471 DOI 10.1128/IAI.00896-18 Anotace Quantitative and microscopic tracking of Borrelia afzelii transmission from infected Ixodes ricinus nymphs has shown a transmission cycle different from that of Borrelia burgdorferi and Ixodes scapularis. Borrelia afzelii organisms are abundant in the guts of unfed I. ricinus nymphs, and their numbers continuously decrease during feeding. Borrelia afzelii spirochetes are present in murine skin within 1 day of tick attachment. In contrast, spirochetes were not detectable in salivary glands at any stage of tick feeding. Further experiments demonstrated that tick saliva is not essential for B. afzelii infectivity, the most important requirement for successful host colonization being a change in expression of outer surface proteins that occurs in the tick gut during feeding. Spirochetes in vertebrate mode are then able to survive within the host even in the absence of tick saliva. Taken together, our data suggest that the tick gut is the decisive organ that determines the competence of I. ricinus to vector B. afzelii. We discuss possible transmission mechanisms of B. afzelii spirochetes that should be further tested in order to design effective preventive and therapeutic strategies against Lyme disease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://iai.asm.org/content/iai/87/6/e00896-18.full.pdf
Počet záznamů: 1