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Oxidative stress in microbes after exposure to iron nanoparticles: analysis of aldehydes as oxidative damage products of lipids and proteins
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SYSNO ASEP 0519320 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Oxidative stress in microbes after exposure to iron nanoparticles: analysis of aldehydes as oxidative damage products of lipids and proteins Tvůrce(i) Semerád, Jaroslav (MBU-M) ORCID, RID
Moeder, M. (DE)
Filip, J. (CZ)
Pivokonský, Martin (UH-J) SAI, ORCID, RID
Filipová, Alena (MBU-M) RID, ORCID
Cajthaml, Tomáš (MBU-M) RID, ORCIDZdroj.dok. Environmental Science and Pollution Research. - : Springer - ISSN 0944-1344
Roč. 26, 32 SI (2019), s. 33670-33682Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Nanomaterial ; Oxidative stress ; Toxicity assay Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Ústav pro hydrodynamiku - Znečištění a kontrola vody CEP TE01020218 GA TA ČR - Technologická agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 ; UH-J - RVO:67985874 UT WOS 000501757400087 EID SCOPUS 85074048363 DOI 10.1007/s11356-019-06370-w Anotace Due to their enhanced reactivity, metal and metal-oxide nanoscale zero-valent iron (nZVI) nanomaterials have been introduced into remediation practice. To ensure that environmental applications of nanomaterials are safe, their possible toxic effects should be described. However, there is still a lack of suitable toxicity tests that address the specific mode of action of nanoparticles, especially for nZVI. This contribution presents a novel approach for monitoring one of the most discussed adverse effects of nanoparticles, i.e., oxidative stress (OS). We optimized and developed an assay based on headspace-SPME-GC-MS analysis that enables the direct determination of volatile oxidative damage products (aldehydes) of lipids and proteins in microbial cultures after exposure to commercial types of nZVI. The method employs PDMS/DVB SPME fibers and pentafluorobenzyl derivatization, and the protocol was successfully tested using representatives of bacteria, fungi, and algae. Six aldehydes, namely, formaldehyde, acrolein, methional, benzaldehyde, glyoxal, and methylglyoxal, were detected in the cultures, and all of them exhibited dose-dependent sigmoidal responses. The presence of methional, which was detected in all cultures except those including an algal strain, documents that nZVI also caused oxidative damage to proteins in addition to lipids. The most sensitive toward nZVI exposure in terms of aldehyde production was the yeast strain Saccharomyces cerevisiae, which had an EC50 value of 0.08 g/L nZVI. To the best of our knowledge, this paper is the first to document the production of aldehydes resulting from lipids and proteins as a result of OS in microorganisms from different kingdoms after exposure to iron nanoparticles. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://link.springer.com/article/10.1007%2Fs11356-019-06370-w
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