Počet záznamů: 1
Deciphering the Origin and Evolution of the X1X2Y System in Two Closely-Related Oplegnathus Species (Oplegnathidae and Centrarchiformes)
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SYSNO ASEP 0517371 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Deciphering the Origin and Evolution of the X1X2Y System in Two Closely-Related Oplegnathus Species (Oplegnathidae and Centrarchiformes) Tvůrce(i) Xu, D. (CN)
Sember, Alexandr (UZFG-Y) RID, ORCID
Zhu, Q. (CN)
de Oliveira, E. A. (BR)
Liehr, T. (DE)
Al-Rikabi, A. B. H. (DE)
Xiao, Z. (CN)
Song, H. (CN)
de Bello Cioffi, M. (BR)Číslo článku 3571 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 20, č. 14 (2019)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova comparative genomic hybridization ; centric fusion ; multiple sex chromosomes Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000480449300195 EID SCOPUS 85070477220 DOI 10.3390/ijms20143571 Anotace Oplegnathus fasciatus and 0. punctatus (Teleostei: Centrarchiformes: Oplegnathidae), are commercially important rocky reef fishes, endemic to East Asia. Both species present an X1X2Y sex chromosome system. Here, we investigated the evolutionary forces behind the origin and differentiation of these sex chromosomes, with the aim to elucidate whether they had a single or convergent origin. To achieve this, conventional and molecular cytogenetic protocols, involving the mapping of repetitive DNA markers, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) were applied. Both species presented similar 2n, karyotype structure and hybridization patterns of repetitive DNA classes. 5S rDNA loci, besides being placed on the autosomal pair 22, resided in the terminal region of the long arms of both X1 chromosomes in females, and on the X1 and Y chromosomes in males. Furthermore, WCP experiments with a probe derived from the Y chromosome of O. fasciatus (OFAS-Y) entirely painted the X1 and X2 chromosomes in females and the X1, X2, and Y chromosomes in males of both species. CGH failed to reveal any sign of sequence differentiation on the Y chromosome in both species, thereby suggesting the shared early stage of neo-Y chromosome differentiation. Altogether, the present findings confirmed the origin of the X1X2Y sex chromosomes via Y-autosome centric fusion and strongly suggested their common origin. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/1422-0067/20/14/3571
Počet záznamů: 1