Počet záznamů: 1
Genome invasion by a hypomethylated satellite repeat in Australian crucifer Ballantinia antipoda
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SYSNO ASEP 0509278 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genome invasion by a hypomethylated satellite repeat in Australian crucifer Ballantinia antipoda Tvůrce(i) Finke, A. (DE)
Mandáková, T. (CZ)
Nawaz, Kashif (UEB-Q)
Vu, G.T.H. (DE)
Novák, Petr (BC-A) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAI
Lysák, M. A. (CZ)
Pečinka, Aleš (UEB-Q) ORCID, RIDCelkový počet autorů 8 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 99, č. 6 (2019), s. 1066-1079Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Brassicaceae ; comparative genomics ; DNA methylation ; heterochromatin ; satellite repeats Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Genetika a molekulární biologie CEP GBP501/12/G090 GA ČR - Grantová agentura ČR LQ1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 ; BC-A - RVO:60077344 UT WOS 000473845500001 EID SCOPUS 85068210358 DOI 10.1111/tpj.14380 Anotace Repetitive sequences are ubiquitous components of all eukaryotic genomes. They contribute to genome evolution and the regulation of gene transcription. However, the uncontrolled activity of repetitive sequences can negatively affect genome functions and stability. Therefore, repetitive DNAs are embedded in a highly repressive heterochromatic environment in plant cell nuclei. Here, we analyzed the sequence, composition and the epigenetic makeup of peculiar non-pericentromeric heterochromatic segments in the genome of the Australian crucifer Ballantinia antipoda. By the combination of high throughput sequencing, graph-based clustering and cytogenetics, we found that the heterochromatic segments consist of a mixture of unique sequences and an A−T-rich 174 bp satellite repeat (BaSAT1). BaSAT1 occupies about 10% of the B. antipoda nuclear genome in >250 000 copies. Unlike many other highly repetitive sequences, BaSAT1 repeats are hypomethylated, this contrasts with the normal patterns of DNA methylation in the B. antipoda genome. Detailed analysis of several copies revealed that these non-methylated BaSAT1 repeats were also devoid of heterochromatic histone H3K9me2 methylation. However, the factors decisive for the methylation status of BaSAT1 repeats remain currently unknown. In summary, we show that even highly repetitive sequences can exist as hypomethylated in the plant nuclear genome. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://dx.doi.org/10.1111/tpj.14380
Počet záznamů: 1