Počet záznamů: 1  

Effects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope

  1. 1.
    SYSNO ASEP0508111
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEffects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope
    Tvůrce(i) Dordevic, B. (CZ)
    Neděla, Vilém (UPT-D) RID, ORCID, SAI
    Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
    Trojan, V. (CZ)
    Havel, L. (CZ)
    Celkový počet autorů5
    Zdroj.dok.New Biotechnology. - : Elsevier - ISSN 1871-6784
    Roč. 48, JAN (2019), s. 35-43
    Poč.str.9 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovacopper ; arsenic ; somatic embryogenesis ; Picea abies (L.) Karst. ; environmental scanning electron microscope
    Vědní obor RIVJA - Elektronika a optoelektronika, elektrotechnika
    Obor OECDPlant sciences, botany
    CEPLO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    GA14-22777S GA ČR - Grantová agentura ČR
    ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaUPT-D - RVO:68081731
    UT WOS000450366200006
    EID SCOPUS85047354110
    DOI10.1016/j.nbt.2018.05.005
    AnotaceSomatic embryogenesis is an important biotechnological technique which can be used in studies associated with environmental stress. Four embryogenic cell lines of Norway spruce were grown on media enriched with copper and arsenic in concentration ranges 50-500 mu M and 10-50 mu M, respectively. The effects were observed during subsequent stages of somatic embryogenesis, the characteristics evaluated being proliferation potential, average number of somatic embryos obtained per g/fresh weight, morphology of developed somatic embryos, metal uptake, and microanalysis of macro-and micronutrients uptake. Copper and arsenic at higher concentrations significantly reduced the growth of early somatic embryos. In almost all treatments, the cell line V-1-3 showed the best performance compared with the other lines tested. Environmental scanning electron microscopy was used to visualize and identify morphological abnormalities in the development of somatic embryos. Abnormalities observed were classified into several categories: meristemless somatic embryos, somatic embryos with disrupted meristem, reduced number of cotyledons, single cotyledon and fused cotyledons. With the application of a low temperature method for the environmental scanning electron microscope, samples were stabilized and whole meristems could be investigated in their native state. As far as we are aware, this is the first report of the effect of copper and arsenic during the process of somatic embryogenesis and the first to evaluate the content of macro and micronutrients uptake in Norway spruce.
    PracovištěÚstav přístrojové techniky
    KontaktMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Rok sběru2020
    Elektronická adresahttps://www.sciencedirect.com/science/article/pii/S1871678417305174?via%3Dihub
Počet záznamů: 1  

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