Počet záznamů: 1
Decreased collagen VI in the tunica media of pulmonary vessels during exposure to hypoxia: a novel step in pulmonary arterial remodeling
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SYSNO ASEP 0507578 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Decreased collagen VI in the tunica media of pulmonary vessels during exposure to hypoxia: a novel step in pulmonary arterial remodeling Tvůrce(i) Žaloudíková, M. (CZ)
Eckhardt, Adam (FGU-C) RID, ORCID
Vytášek, R. (CZ)
Uhlík, J. (CZ)
Novotný, T. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Musílková, Jana (FGU-C) RID, ORCID
Hampl, V. (CZ)Číslo článku 2045894019860747 Zdroj.dok. Pulmonary Circulation. - : Wiley - ISSN 2045-8932
Roč. 9, č. 3 (2019)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova pulmonary arterial remodeling ; collagen VI ; tunica media ; smooth muscle cells ; hypoxic pulmonary hypertension Vědní obor RIV FC - Pneumologie Obor OECD Respiratory systems CEP NV17-31564A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000475324000001 EID SCOPUS 85068965572 DOI 10.1177/2045894019860747 Anotace The development of hypoxic pulmonary hypertension is characterized by the structural remodeling of pulmonary arteries. However, the relationship between changes of arterial cells and the extracellular matrix remains unclear. We focused on the evaluation of the non-fibrillar collagen changes in tunica media induced by a four-day exposure to hypoxia and the correlation of these changes with the pulmonary arterial wall structure modifications. We used 20 adult male Wistar rats. The amount and localization of collagen VI, collagen IV, matrix metalloproteinase (MMP) 2, and MMP9 were tested in pulmonary arteries immunohistochemically. Two-dimensional electrophoresis and messenger RNA (mRNA) expression were used for the subsequent comparison of protein changes in arterial tunica media cells (normoxia/hypoxia). Collagen VI was significantly reduced strictly in the tunica media of conduit arteries of hypoxia-exposed rats, however, its mRNA increased. The amount of collagen IV and its mRNA were not altered. We detected a significant increase of MMP9 strictly in the tunica media. In addition, a significantly increased number of MMP9-positive cells surrounded the arteries. MMP2 and the expression of its mRNA were decreased in tunica media. We conclude that the loss of collagen VI is an important step characterizing the remodeling of pulmonary arteries. It could influence the phenotypic status and behavior of smooth muscle cells and modify their proliferation and migration. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1177/2045894019860747
Počet záznamů: 1