Počet záznamů: 1
Dose-Rate Effects in Breaking DNA Strands by Short Pulses of Extreme Ultraviolet Radiation
- 1.
SYSNO ASEP 0506556 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dose-Rate Effects in Breaking DNA Strands by Short Pulses of Extreme Ultraviolet Radiation Tvůrce(i) Vyšín, L. (CZ)
Burian, Tomáš (UFCH-W) ORCID, RID, SAI
Ukraintsev, E. (CZ)
Davídková, M. (CZ)
Grisham, M. E. (US)
Heinbuch, S. (US)
Rocca, J.J. (US)
Juha, L. (CZ)Zdroj.dok. Radiation Research. - : Radiation Research Society - ISSN 0033-7587
Roč. 189, č. 5 (2018), s. 466-476Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova soft-x-ray ; low-energy electrons ; plasmid dna ; single-strand ; adenine residues ; photon energy ; abasic sites Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GA13-28721S GA ČR - Grantová agentura ČR GBP108/12/G108 GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000430572800003 EID SCOPUS 85045934898 DOI 10.1667/RR14825.1 Anotace In this study, we examined dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single-and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. In addition, electrophoresis also revealed DNA cross-linking. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. The average SSB/DSB ratio decreased only slightly at elevated dose rates. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study. (C) 2018 by Radiation Research Society. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2020 Elektronická adresa http://hdl.handle.net/11104/0297789
Počet záznamů: 1