Počet záznamů: 1
Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids
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SYSNO ASEP 0505428 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids Tvůrce(i) Béres, T. (CZ)
Černochová, L. (CZ)
Ćavar Zeljković, S. (CZ)
Benická, S. (CZ)
Gucký, Tomáš (UEB-Q) ORCID
Berčák, M. (CZ)
Tarkowski, P. (CZ)Celkový počet autorů 7 Zdroj.dok. Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 411, č. 14 (2019), s. 3069-3079Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Flame ionization detector ; Gas chromatography ; Liquid chromatography ; Mass spectrometry ; Phytocannabinoids Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000468293800008 EID SCOPUS 85063188200 DOI 10.1007/s00216-019-01760-y Anotace This study compares alternative approaches for analyzing phytocannabinoids in different plant materials. Three chromatographic analytical methods (ultra-high-performance liquid chromatography with tandem mass spectrometric detection and gas chromatography with mass spectrometric and flame ionization detection) were evaluated regarding selectivity, sensitivity, analytical accuracy, and precision. The performance of the methods was compared and all three methods were demonstrated to be appropriate tools for analyzing phytocannabinoids in cannabis. Gas chromatography coupled with mass spectrometric detection showed slightly better accuracy in determining phytocannabinoid acids, which are often difficult to quantify owing to their limited stability. Aspects of sample preparation, such as material homogenization and extraction, were also considered. A single ultrasonic-assisted ethanolic extraction of dried and powdered plant samples of cannabis was shown to be exhaustive for extracting the samples prior to analysis. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://doi.org/10.1007/s00216-019-01760-y
Počet záznamů: 1