Počet záznamů: 1
Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon
- 1.
SYSNO ASEP 0504502 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon Tvůrce(i) Čepková Hlásná, P. (CZ)
Jágr, Michal (FGU-C) RID, ORCID
Viehmannová, I. (CZ)
Dvořáček, V. (CZ)
Huansi, D. C. (PE)
Mikšík, Ivan (FGU-C) RID, ORCID, SAIZdroj.dok. International Journal of Agriculture and Biology. - : Friends Science Publishers - ISSN 1560-8530
Roč. 21, č. 3 (2019), s. 679-688Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. PK - Pákistán Klíč. slova genetic variability ; oilseeds ; proteomics ; storage proteins Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA15-01948S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000456544000026 EID SCOPUS 85062499141 Anotace Sacha inchi (Plukenetia volubilis L.) is a plant native to the Peruvian Amazon and produces seeds rich in some nutraceutical compounds with a high protein content. The present work aimed to characterize P. volubilis seed proteins and compare their proteomic profile between different P. volubilis populations. Crude protein content in the seeds was detected at a mean level of 22.56% with the major proportion of albumins and globulins (16.37%), followed by glutelins (5.87%) and a very low content of prolamins (0.33%). Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of both total seed flour and Osborne's protein fractions of different populations showed that proteins were concentrated in the 8-75 kDa range. Differences in abundance of proteins between Sacha inchi populations were detected by SD S-PAGE and two-dimensional (2-DE) gel electrophoresis. Gel protein bands and spots of interest were excised, digested with trypsin and analyzed by nano-liquid chromatography/tandem mass spectrometry (nLC-MS/MS). This is the first direct identification of these proteins in actual Sacha inchi seeds. Three proteins (Oleosin 2, Oleosin 3 and elongation factor 1-alpha) directly from P. volubilis and peptides with a sequence identical to 56 proteins from the Euphothiaceae family were detected. This characterization of seed storage protein, their variability within populations and identification of important proteins provide a basis for further investigations for the food industry and bioengineering. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa http://www.fspublishers.org/published_papers/23739_26%20doi%2015.0945%20IJAB-18-1080%20(10)%20679-688.pdf
Počet záznamů: 1