Počet záznamů: 1
Microbiomes of North American triatominae: The grounds for Chagas disease epidemiology
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SYSNO ASEP 0498800 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microbiomes of North American triatominae: The grounds for Chagas disease epidemiology Tvůrce(i) Rodríguez-Ruano, S. M. (CZ)
Škochová, V. (CZ)
Rego, Ryan O. M. (BC-A) RID, ORCID
Schmidt, J. O. (US)
Roachell, W. (US)
Hypša, Václav (BC-A) RID
Nováková, Eva (BC-A) RID, ORCIDCelkový počet autorů 7 Číslo článku 1167 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 9, JUN (2018)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Microbiome ; Ontogeny ; Rhodnius prolixus ; Triatominae ; Trypanosoma cruzi Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Institucionální podpora BC-A - RVO:60077344 UT WOS 000435093000001 EID SCOPUS 85048579089 DOI 10.3389/fmicb.2018.01167 Anotace Insect microbiomes influence many fundamental host traits, including functions of practical significance such as their capacity as vectors to transmit parasites and pathogens. The knowledge on the diversity and development of the gut microbiomes in various blood feeding insects is thus crucial not only for theoretical purposes, but also for the development of better disease control strategies. In Triatominae (Heteroptera: Reduviidae), the blood feeding vectors of Chagas disease in South America and parts of North America, the investigation of the microbiomes is in its infancy. The few studies done on microbiomes of South American Triatominae species indicate a relatively low taxonomic diversity and a high host specificity. We designed a comparative survey to serve several purposes: (I) to obtain a better insight into the overall microbiome diversity in different species, (II) to check the long term stability of the interspecific differences, (III) to describe the ontogenetic changes of the microbiome, and (IV) to determine the potential correlation between microbiome composition and presence of Trypanosoma cruzi, the causative agent of Chagas disease. Using 16S amplicons of two abundant species from the southern US, and four laboratory reared colonies, we showed that the microbiome composition is determined by host species, rather than locality or environment. The OTUs (Operational Taxonomic Units) determination confirms a low microbiome diversity, with 12-17 main OTUs detected in wild populations of T. sanguisuga and T. protracta. Among the dominant bacterial taxa are Acinetobacter and Proteiniphilum but also the symbiotic bacterium Arsenophonus triatominarum, previously believed to only live intracellularly. The possibility of ontogenetic microbiome changes was evaluated in all six developmental stages and feces of the laboratory reared model Rhodnius prolixus. We detected considerable changes along the host's ontogeny, including clear trends in the abundance variation of the three dominant bacteria, namely Enterococcus, Acinetobacter, and Arsenophonus. Finally, we screened the samples for the presence of Trypanosoma cruzi. Comparing the parasite presence with the microbiome composition, we assessed the possible significance of the latter in the epidemiology of the disease. Particularly, we found a trend toward more diverse microbiomes in Trypanosoma cruzi positive T. protracta specimens. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2019 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2018.01167/full
Počet záznamů: 1