Počet záznamů: 1
Antiplasmodial activity of tick defensins in a mouse model of malaria
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SYSNO ASEP 0498052 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antiplasmodial activity of tick defensins in a mouse model of malaria Tvůrce(i) Couto, A.S. (AR)
Tonk, M. (DE)
Ferrolho, J. (GB)
Antunes, S. (PT)
Vilcinskas, A. (DE)
de la Fuente, J. (ES)
Domingos, A. (PT)
Cabezas Cruz, Alejandro (BC-A)Celkový počet autorů 8 Zdroj.dok. Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 9, č. 4 (2018), s. 844-849Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Malaria ; Plasmodium ; Defensin ; Tick ; Antiplasmodial activity Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology Institucionální podpora BC-A - RVO:60077344 UT WOS 000433129600015 EID SCOPUS 85044099359 DOI 10.1016/j.ttbdis.2018.03.011 Anotace Malaria is a mosquito-borne disease affecting millions of people mainly in Sub-Saharan Africa, Asia and some South American countries. Drug resistance to first-line antimalarial drugs (e.g. chloroquine, sulfadoxine-pyrimethamine and artemisinin) is a major constrain in malaria control. Antimicrobial peptides (AMPs) have shown promising results in controlling Plasmodium spp. parasitemia in in vitro and in vivo models of infection. Defensins are AMPs that act primarily by disrupting the integrity of cell membranes of invasive microbes. We previously showed that defensins from the tick Ixodes ricinus inhibited significantly the growth of P. falciparum in vitro, a property that was conserved during evolution. Here, we tested the activity of three I. ricinus defensins against P. chabaudi in mice. A single dose of defensin (120 mu l of 1 mg/ml solution) was administered intravenously to P. chabaudi-infected mice, and the parasitemia was followed for 24 h post-treatment. Defensin treatment inhibited significantly the replication (measured as increases in parasitemia) of P. chabaudi after 1 h and 12 h of treatment. Furthermore, defensin injection was not associated with toxicity. These results agreed with the previous report of antiplasmodial activity of tick defensins against P. falciparum in vitro and justify further studies for the use of tick defensins to control malaria. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2019
Počet záznamů: 1