Počet záznamů: 1
Gene expression profiling in healthy newborns from diverse localities of the Czech Republic
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SYSNO ASEP 0493340 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gene expression profiling in healthy newborns from diverse localities of the Czech Republic Tvůrce(i) Hoňková, Kateřina (UEM-P)
Rössnerová, Andrea (UEM-P) RID
Pavlíková, Jitka (UEM-P) ORCID
Švecová, Vlasta (UEM-P) RID
Kléma, J. (CZ)
Topinka, Jan (UEM-P) RID, ORCID
Milcová, Alena (UEM-P)
Líbalová, Helena (UEM-P) RID
Choi, H. (US)
Veleminský Jr., M. (CZ)
Šrám, Radim (UEM-P) RID
Rössner ml., Pavel (UEM-P) RID, ORCIDZdroj.dok. Environmental and Molecular Mutagenesis. - : Wiley - ISSN 0893-6692
Roč. 59, č. 5 (2018), s. 401-415Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova air pollution ; benzo[a]pyrene ; cord blood ; microarray Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA13-13458S GA ČR - Grantová agentura ČR LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000437277600004 EID SCOPUS 85044619108 DOI 10.1002/em.22184 Anotace Prenatal exposure to air pollution is associated with intrauterine growth restriction and low birth weight. Gene expression changes in newborns in relation to air pollution have not been sufficiently studied. We analyzed whole genome expression in cord blood leukocytes of 202 newborns from diverse localities of the Czech Republic, differing among other factors in levels of air pollution: the district of Karvina (characterized by higher concentration of air pollutants) and Ceske Budejovice (lower air pollution levels). We aimed to identify differentially expressed genes (DEGs) and pathways in relation to locality and concentration of air pollutants. We applied the linear model to identify the specific DEGs and the correlation analysis, to investigate the relationship between the concentrations of air pollutants and gene expression data. An analysis of biochemical pathways and gene set enrichment was also performed. In general, we observed modest changes of gene expression, mostly attributed to the effect of the locality. The highest number of DEGs was found in samples from the district of Karvina. A pathway analysis revealed a deregulation of processes associated with cell growth, apoptosis or cellular homeostasis, immune response-related processes or oxidative stress response. The association between concentrations of air pollutants and gene expression changes was weak, particularly for samples collected in Karvina. In summary, as we did not find a direct effect of exposure to air pollutants, we assume that the general differences in the environment, rather than actual concentrations of individual pollutants, represent a key factor affecting gene expression changes at delivery. Environ. Mol. Mutagen. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
Počet záznamů: 1