Počet záznamů: 1
Dynamics of tissue ingrowth in SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores after bridging a spinal cord transection
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SYSNO ASEP 0492877 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dynamics of tissue ingrowth in SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores after bridging a spinal cord transection Tvůrce(i) Hejčl, Aleš (UEM-P) RID, ORCID
Růžička, Jiří (UEM-P)
Proks, Vladimír (UMCH-V) RID, ORCID
Macková, Hana (UMCH-V) RID
Kubinová, Šárka (UEM-P) RID, ORCID
Tukmachev, D. (CZ)
Cihlář, J. (CZ)
Horák, Daniel (UMCH-V) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCIDČíslo článku 89 Zdroj.dok. Journal of Materials Science-Materials in Medicine. - : Springer - ISSN 0957-4530
Roč. 29, č. 7 (2018)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova mesenchymal STEM-CELLS ; sensory axon regenerationsensory axon regeneration ; endothelial growth-factor ; functional recovery Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP GA14-14961S GA ČR - Grantová agentura ČR GA17-11140S GA ČR - Grantová agentura ČR LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF15_003/0000419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000436341100002 EID SCOPUS 85049203497 DOI 10.1007/s10856-018-6100-2 Anotace While many types of biomaterials have been evaluated in experimental spinal cord injury (SCI) research, little is known about the time-related dynamics of the tissue infiltration of these scaffolds. We analyzed the ingrowth of connective tissue, axons and blood vessels inside the superporous poly (2-hydroxyethyl methacrylate) hydrogel with oriented pores. The hydrogels, either plain or seeded with mesenchymal stem cells (MSCs), were implanted in spinal cord transection at the level of Th8. The animals were sacrificed at days 2, 7, 14, 28, 49 and 6 months after SCI and histologically evaluated. We found that within the first week, the hydrogels were already infiltrated with connective tissue and blood vessels, which remained stable for the next 6 weeks. Axons slowly and gradually infiltrated the hydrogel within the first month, after which the numbers became stable. Six months after SCI we observed rare axons crossing the hydrogel bridge and infiltrating the caudal stump. There was no difference in the tissue infiltration between the plain hydrogels and those seeded with MSCs. We conclude that while connective tissue and blood vessels quickly infiltrate the scaffold within the first week, axons show a rather gradual infiltration over the first month, and this is not facilitated by the presence of MSCs inside the hydrogel pores. Further research which is focused on the permissive micro-environment of the hydrogel scaffold is needed, to promote continuous and long-lasting tissue regeneration across the spinal cord lesion. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
Počet záznamů: 1