Počet záznamů: 1
CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization
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SYSNO ASEP 0492845 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization Tvůrce(i) Frolíková, Michaela (BTO-N) ORCID
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Černý, Jiří (BTO-N) RID, ORCID
Jankovičová, J. (SK)
Šimoník, Ondřej (BTO-N)
Pohlová, Alžběta (BTO-N)
Sečová, P. (SK)
Antalíková, J. (SK)
Dvořáková-Hortová, Kateřina (BTO-N)Celkový počet autorů 9 Číslo článku 1236 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 4 (2018)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova TETRASPANIN-ENRICHED MICRODOMAINS ; ACROSOME REACTION ; LIPID RAFTS Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 UT WOS 000434978700315 EID SCOPUS 85045909159 DOI 10.3390/ijms19041236 Anotace Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm-egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9/CD81 double null female mice. In this study, the putative mechanism of CD9/CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9/CD81 localization visualised in detail by super-resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosome reaction in human sperm, only CD9 is relocated, compared to the relocation of both proteins in mice. The structural modelling of CD9 and CD81 homologous and possibly heterologous network formation was used to propose their lateral Cis as well as Trans interactions within the sperm membrane and during sperm-egg membrane fusion. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2019
Počet záznamů: 1