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Proteomic analysis of cardiac ventricles: baso-apical differences
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SYSNO ASEP 0491342 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proteomic analysis of cardiac ventricles: baso-apical differences Tvůrce(i) Eckhardt, Adam (FGU-C) RID, ORCID
Kulhavá, Lucie (FGU-C) ORCID
Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Pataridis, Statis (FGU-C)
Hlaváčková, Markéta (FGU-C) RID, ORCID
Vašinová, Jana (FGU-C)
Kolář, František (FGU-C) RID, ORCID, SAI
Sedmera, David (FGU-C) RID, ORCID, SAI
Ošťádal, Bohuslav (FGU-C) RID, ORCIDZdroj.dok. Molecular and Cellular Biochemistry. - : Springer - ISSN 0300-8177
Roč. 445, 1-2 (2018), s. 211-219Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova proteomics ; heart ; ventricle ; myocardial heterogeneity ; two-dimensional electrophoresis ; ventricular myocardium Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GJ16-12420Y GA ČR - Grantová agentura ČR GA16-02972S GA ČR - Grantová agentura ČR GA13-12412S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000437464800021 EID SCOPUS 85040044865 DOI 10.1007/s11010-017-3266-8 Anotace The heart is characterized by a remarkable degree of heterogeneity. Since different cardiac pathologies affect different cardiac regions, it is important to understand molecular mechanisms by which these parts respond to pathological stimuli. In addition to already described left ventricular (LV)/right ventricular (RV) and transmural differences, possible baso-apical heterogeneity has to be taken into consideration. The aim of our study has been, therefore, to compare proteomes in the apical and basal parts of the rat RV and LV. Two-dimensional electrophoresis was used for the proteomic analysis. The major result of this study has revealed for the first time significant baso-apical differences in concentration of several proteins, both in the LV and RV. As far as the LV is concerned, five proteins had higher concentration in the apical compared to basal part of the ventricle. Three of them are mitochondrial and belong to the “metabolism and energy pathways” (myofibrillar creatine kinase M-type, l-lactate dehydrogenase, dihydrolipoamide dehydrogenase). Myosin light chain 3 is a contractile protein and HSP60 belongs to heat shock proteins. In the RV, higher concentration in the apical part was observed in two mitochondrial proteins (creatine kinase S-type and proton pumping NADH:ubiquinone oxidoreductase). The described changes were more pronounced in the LV, which is subjected to higher workload. However, in both chambers was the concentration of proteins markedly higher in the apical than that in basal part, which corresponds to the higher energetic demand and contractile activity of these segments of both ventricles. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
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