Počet záznamů: 1
Endocardial Fibroelastosis is Secondary to Hemodynamic Alterations in the Chick Embryonic Model of Hypoplastic Left Heart Syndrome
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SYSNO ASEP 0490360 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Endocardial Fibroelastosis is Secondary to Hemodynamic Alterations in the Chick Embryonic Model of Hypoplastic Left Heart Syndrome Tvůrce(i) Peševski, Živorad (FGU-C)
Kvasilová, A. (CZ)
Stopková, T. (CZ)
Naňka, O. (CZ)
Drobná Krejčí, Eliška (FGU-C)
Buffinton, Ch. (US)
Kočková, Radka (FGU-C)
Eckhardt, Adam (FGU-C) RID, ORCID
Sedmera, David (FGU-C) RID, ORCID, SAIZdroj.dok. Developmental Dynamics. - : Wiley - ISSN 1058-8388
Roč. 247, č. 3 (2018), s. 509-520Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova hypoxia ; HLHS ; left atrial ligation ; collagen ; chick embryo Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Anatomy and morphology (plant science to be 1.6) CEP GAP302/11/1308 GA ČR - Grantová agentura ČR GA16-02972S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000425141200016 EID SCOPUS 85020829846 DOI 10.1002/dvdy.24521 Anotace Endocardial fibroelastosis (EFE) is a diffuse thickening of the ventricular endocardium, causing myocardial dysfunction and presenting as unexplained heart failure in infants and children. One of the postulated causes is persistent and increased wall tension in the ventricles. Results: To examine whether reduced ventricular pressure in a chick model of hypoplastic left heart syndrome (HLHS) induced by left atrial ligation (LAL) at embryonic day (ED) 4 is associated with EFE at later stages, myocardial fibrosis was evaluated by histology and immunoconfocal microscopy and mass spectrometry (MS) at ED12. Immunohistochemistry with collagen I antibody clearly showed a significant thickening of the layer of subendocardial fibrous tissue in LAL hearts, and MS proved this significant increase of collagen I. To provide further insight into pathogenesis of this increased fibroproduction, hypoxyprobe staining revealed an increased extent of hypoxic regions, normally limited to the interventricular septum, in the ventricular myocardium of LAL hearts at ED8. Conclusions: Abnormal hemodynamic loading during heart development leads to myocardial hypoxia, stimulating collagen production in the subendocardium. Therefore, EFE in this chick embryonic model of HLHS appears to be a secondary effect of abnormal hemodynamics. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
Počet záznamů: 1