Počet záznamů: 1

Mitochondrion remodeling during T. b. brucei developmental differentiation

SYSNO ASEP	0488338
Druh ASEP	O - Ostatní výsledky
Zařazení RIV	O - Ostatní
Název	Mitochondrion remodeling during T. b. brucei developmental differentiation
Tvůrce(i)	Doleželová, Eva (BC-A)_{RID, ORCID} Panicucci, Brian (BC-A) Zíková, Alena (BC-A)_{RID, ORCID}
Rok vydání	2017
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	mitochondrion remodeling ; developmental differentiation ; T. brucei
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Biochemistry and molecular biology
CEP	LL1205 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BC-A - RVO:60077344
Anotace	Trypanosoma brucei undergoes a complex life cycle as it alternates between a mammalian host and the blood-feeding insect vector, a tsetse fly. Due to the different environments, the distinct life stages differ in their energy metabolism, i.e. insect stage (procyclic cells, PS) depends on mitochondrial oxidative phosphorylation (OXPHOS) for ATP production while the bloodstream stage (BS) gains energy by aerobic glycolysis. The dramatic switch from the OXPHOS to glycolysis happens during the complex development of the PS in the tsetse fly. This development differentiation is characterized by extensive remodeling of mitochondrion structure and changes in mitochondrial bioenergetics. Importantly, the molecular mechanism behind this process is completely unknown. We have established the in vitro differentiation system, in which the transition from PS to epimastigotes followed by differentiation to transmission-ready metacylic trypanosomes is triggered by RNA binding protein 6 (RBP6) expression. This in vitro induced differentiation of PF cells takes 8 days. The appearance of epimastigotes and metacyclic trypanosomes in the culture was mapped using light and fluorescent microscopy. The whole cell proteome of cell culture harvested every day after the RBP6 induction was identified by label-free quantitative mass spectrometry. This proteomic data serves as a resource for further detailed characterization of changes happening in the parasite mitochondrion as well as identification of possible candidates involved in the PS differentiation.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2018

Počet záznamů: 1