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Unique double concentric ring organization of light harvesting complexes in Gemmatimonas phototrophica
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SYSNO ASEP 0484727 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Unique double concentric ring organization of light harvesting complexes in Gemmatimonas phototrophica Tvůrce(i) Dachev, Marko (MBU-M) ORCID
Bína, David (BC-A) RID, ORCID
Sobotka, Roman (MBU-M) RID, ORCID
Moravcová, Lenka (MBU-M)
Gardian, Zdenko (BC-A) RID
Kaftan, David (MBU-M) ORCID
Šlouf, V. (CZ)
Fuciman, M. (CZ)
Polívka, Tomáš (BC-A) RID, ORCID
Koblížek, Michal (MBU-M) RID, ORCIDČíslo článku e2003943 Zdroj.dok. PLOS Biology. - : Public Library of Science - ISSN 1544-9173
Roč. 15, č. 12 (2017)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova BACTERIUM CHLOROFLEXUS-AURANTIACUS ; EXCITATION-ENERGY TRANSFER ; PURPLE BACTERIA Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Biofyzika CEP GA15-00703S GA ČR - Grantová agentura ČR GBP501/12/G055 GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000418943900019 EID SCOPUS 85039903888 DOI 10.1371/journal.pbio.2003943 Anotace The majority of life on Earth depends directly or indirectly on the sun as a source of energy. The initial step of photosynthesis is facilitated by light-harvesting complexes, which capture and transfer light energy into the reaction centers (RCs). Here, we analyzed the organization of photosynthetic (PS) complexes in the bacterium G. phototrophica, which so far is the only phototrophic representative of the bacterial phylum Gemmatimonadetes. The isolated complex has a molecular weight of about 800 +/- 100 kDa, which is approximately 2 times larger than the core complex of Rhodospirillum rubrum. The complex contains 62.4 +/- 4.7 bacteriochlorophyll (BChl) a molecules absorbing in 2 distinct infrared absorption bands with maxima at 816 and 868 nm. Using femtosecond transient absorption spectroscopy, we determined the energy transfer time between these spectral bands as 2 ps. Single particle analyses of the purified complexes showed that they were circular structures with an outer diameter of approximately 18 nm and a thickness of 7 nm. Based on the obtained, we propose that the LH complexes in G. phototrophica form 2 concentric rings surrounding the type 2 RC. The inner ring (corresponding to the B868 absorption band) is composed of 15 subunits and is analogous to the inner light-harvesting complex 1 (LH1) in purple bacteria. The outer ring is composed of 15 more distant BChl dimers with no or slow energy transfer between them, resulting in the B816 absorption band. This completely unique and elegant organization offers good structural stability, as well as high efficiency of light harvesting. Our results reveal that while the PS apparatus of Gemmatimonadetes was acquired via horizontal gene transfer from purple bacteria, it later evolved along its own pathway, devising a new arrangement of its light harvesting complexes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
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