Počet záznamů: 1
Long non-coding RNA exchange during the oocyte-to-embryo transition in mice
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SYSNO ASEP 0483739 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Long non-coding RNA exchange during the oocyte-to-embryo transition in mice Tvůrce(i) Karlic, R. (HR)
Ganesh, Sravya (UMG-J)
Franke, V. (HR)
Svobodová, Eliška (UMG-J)
Urbanová, Jana (UMG-J)
Suzuki, Y. (JP)
Aoki, F. (JP)
Vlahovicek, K. (HR)
Svoboda, Petr (UMG-J) RIDCelkový počet autorů 9 Zdroj.dok. Dna Research. - : Oxford University Press - ISSN 1340-2838
Roč. 24, č. 2 (2017), s. 129-141Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova IncRNA ; oocyte ; zygote ; polyadenylation ; endo-siRNA Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Reproductive biology (medical aspects to be 3) CEP GBP305/12/G034 GA ČR - Grantová agentura ČR LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2011032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015040 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000400913000003 DOI 10.1093/dnares/dsw058 Anotace The oocyte-to-embryo transition (OET) transforms a differentiated gamete into pluripotent blastomeres. The accompanying maternal-zygotic RNA exchange involves remodeling of the long non-coding RNA (lncRNA) pool. Here, we used next generation sequencing and de novo transcript assembly to define the core population of 1,600 lncRNAs expressed during the OET (lncRNAs). Relative to mRNAs, OET lncRNAs were less expressed and had shorter transcripts, mainly due to fewer exons and shorter 5' terminal exons. Approximately half of OET lncRNA promoters originated in retrotransposons suggesting their recent emergence. Except for a small group of ubiquitous lncRNAs, maternal and zygotic lncRNAs formed two distinct populations. The bulk of maternal lncRNAs was degraded before the zygotic genome activation. Interestingly, maternal lncRNAs seemed to undergo cytoplasmic polyadenylation observed for dormant mRNAs. We also identified lncRNAs giving rise to trans-acting short interfering RNAs, which represent a novel lncRNA category. Altogether, we defined the core OET lncRNA transcriptome and characterized its remodeling during early development. Our results are consistent with the notion that rapidly evolving lncRNAs constitute signatures of cells-of-origin while a minority plays an active role in control of gene expression across OET. Our data presented here provide an excellent source for further OET lncRNA studies. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1