Počet záznamů: 1
Non-aqueous capillary electrophoretic separation of cholesterol and 25-hydroxycholesterol after derivatization with Girard P reagent
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SYSNO ASEP 0480519 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Non-aqueous capillary electrophoretic separation of cholesterol and 25-hydroxycholesterol after derivatization with Girard P reagent Tvůrce(i) Greguš, M. (CZ)
Roberg-Larsen, H. (NO)
Lundanes, E. (NO)
Foret, František (UIACH-O) RID, ORCID
Kubáň, Petr (UIACH-O) ORCID
Wilson, S.R. (NO)Celkový počet autorů 6 Zdroj.dok. Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084
Roč. 207, OCT (2017), s. 87-91Poč.str. 5 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova cholesterol ; 25-hydroxycholesterol ; UV detection Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000412967000006 EID SCOPUS 85020414243 DOI 10.1016/j.chemphyslip.2017.05.014 Anotace Capillary electrophoresis (CE) can provide high separation efficiency with very simple instrumentation, but has yet to be explored regarding oxysterols/cholesterol. Cholesterol and 25-hydroxycholesterol (both are 4-ene-3-ketosteroids) were quantitatively transformed into hydrazones using Girard P reagent after enzymatic oxidation by cholesterol oxidase. Separation was achieved using non-aqueous capillary electrophoresis with UV detection at 280 nm, the charge-tagging Girard P reagent ensured both charge and chromophore (which are requirements for CE-UV). Excess reagent was also separated from the two analytes, eliminating the need for removal prior to the analysis. The compounds were separated in less than 5 min with excellent separation efficiency, using separation electrolytes fully compatible with mass spectrometry. The CE-UV method was used to optimize steps for charge-tagging, revealing that the procedure is affected by the analyte/reagent ratio and reaction time, but also the analyte structure. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2018
Počet záznamů: 1