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Differential effects of carbohydrates on arabidopsis pollen germination
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SYSNO ASEP 0475306 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differential effects of carbohydrates on arabidopsis pollen germination Tvůrce(i) Hirsche, J. (DE)
Fernández, J. M. G. (ES)
Stabentheiner, E. (AT)
Großkinsky, D.K. (DE)
Roitsch, Thomas (UEK-B) RID, ORCID, SAIZdroj.dok. Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 58, č. 4 (2017), s. 691-701Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova Arabidopsis thaliana ; Carbohydrates ; Metabolic regulation ; Pollen germination ; Signaling ; Structure-function relationship Vědní obor RIV EF - Botanika Obor OECD Cell biology CEP LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000402074100006 EID SCOPUS 85019730089 DOI 10.1093/pcp/pcx020 Anotace Pollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of the impact of 32 different sugars on in vitro germination of Arabidopsis pollen comprising about 150 variations of individual sugars and combinations. Twenty-six structurally different mono-, di- and oligosaccharides, and sugar analogs were initially tested for their ability to support pollen germination. Whereas several di- and oligosaccharides supported pollen germination, hexoses such as glucose, fructose and mannose did not support and even considerably inhibited pollen germination when added to germination-supporting medium. Complementary experiments using glucose analogs with varying functional features, the hexokinase inhibitor mannoheptulose and the glucose-insensitive hexokinase-deficient Arabidopsis mutant gin2-1 suggested that mannose- and glucose-mediated inhibition of sucrose-supported pollen germination depends partially on hexokinase signaling. The results suggest that, in addition to their role as energy source, sugars act as signaling molecules differentially regulating the complex process of pollen germination depending on their structural properties. Thus, a sugar-dependent multilayer regulation of Arabidopsis pollen germination is supported, which makes this approach a valuable experimental system for future studies addressing sugar sensing and signaling. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2018
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