Počet záznamů: 1
Pleomorphism and Viability of the Lyme Disease Pathogen Borrelia burgdorferi Exposed to Physiological Stress Conditions: A Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy Study
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SYSNO ASEP 0474412 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Pleomorphism and Viability of the Lyme Disease Pathogen Borrelia burgdorferi Exposed to Physiological Stress Conditions: A Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy Study Tvůrce(i) Vancová, Marie (BC-A) RID, ORCID
Rudenko, Natalia (BC-A) RID, ORCID
Vaněček, Jiří (BC-A)
Golovchenko, Maryna (BC-A) RID, ORCID
Strnad, Martin (BC-A) ORCID
Rego, Ryan O. M. (BC-A) RID, ORCID
Tichá, Lucie (BC-A)
Grubhoffer, Libor (BC-A) RID, ORCID
Nebesářová, Jana (BC-A) RID, ORCIDCelkový počet autorů 9 Číslo článku 596 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 8, 11 April (2017)Poč.str. 9 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova cryo-fluorescence ; cryo-scanning electron microscopy ; Borrelia burgdorferi ; Lyme disease ; round body ; pleomorphism ; viability staining Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD 2.11 Other engineering and technologies CEP TE01020118 GA TA ČR - Technologická agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 UT WOS 000398768200001 EID SCOPUS 85018264201 DOI 10.3389/fmicb.2017.00596 Anotace To understand the response of the Lyme disease spirochete Borrelia burgdorferi exposed to stress conditions and assess the viability of this spirochete, we used a correlative cryo-fluorescence and cryo-scanning microscopy approach. This approach enables simple exposition of bacteria to various experimental conditions that can be stopped at certain time intervals by cryo-immobilization, examination of cell viability without necessity to maintain suitable culture conditions during viability assays, and visualization of structures in their native state at high magnification. We focused on rare and transient events e.g., the formation of round bodies and the presence of membranous blebs in spirochetes exposed to culture medium, host sera either without or with the bacteriolytic effect and water. We described all crucial steps of the workflow, particularly the influence of freeze-etching and accelerating voltage on the visualization of topography. With the help of newly designed cryo-transport device, we achieved greater reproducibility. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1