Počet záznamů: 1
The cyanobacterial metabolite nocuolin a is a natural oxadiazine that triggers apoptosis in human cancer cells.
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SYSNO ASEP 0474362 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The cyanobacterial metabolite nocuolin a is a natural oxadiazine that triggers apoptosis in human cancer cells. Tvůrce(i) Voráčová, Kateřina (MBU-M)
Hájek, Jan (MBU-M) ORCID
Mareš, Jan (MBU-M) ORCID
Urajová, Petra (MBU-M)
Kuzma, Marek (MBU-M) ORCID, RID
Cheel Horna, José (MBU-M)
Villunger, A. (AT)
Kapuscik, Alexandra (MBU-M)
Bally, M. (CA)
Novák, Petr (MBU-M) RID, ORCID
Kabeláč, M. (CZ)
Krumschnabel, G. (AT)
Lukeš, Martin (MBU-M) ORCID
Voloshko, L. (RU)
Kopecký, Jiří (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCIDČíslo článku e0172850 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 3 (2017), s. 1-12Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ENZYMES ; 1,3,4-OXADIAZOLE ; SCAFFOLD Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GPP503/12/P614 GA ČR - Grantová agentura ČR GA14-18067S GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.30.0059 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000396011300042 EID SCOPUS 85014240038 DOI 10.1371/journal.pone.0172850 Anotace Oxadiazines are heterocyclic compounds containing N-N-O or N-N-C-O system within a six membered ring. These structures have been up to now exclusively prepared via organic synthesis. Here, we report the discovery of a natural oxadiazine nocuolin A (NoA) that has a unique structure based on 1,2,3-oxadiazine. We have identified this compound in three independent cyanobacterial strains of genera Nostoc, Nodularia, and Anabaena and recognized the putative gene clusters for NoA biosynthesis in their genomes. Its structure was characterized using a combination of NMR, HRMS and FTIR methods. The compound was first isolated as a positive hit during screening for apoptotic inducers in crude cyanobacterial extracts. We demonstrated that NoA-induced cell death has attributes of caspase-dependent apoptosis. Moreover, NoA exhibits a potent anti-proliferative activity (0.7-4.5 micro) against several human cancer lines, with p53-mutated cell lines being even more sensitive. Since cancers bearing p53 mutations are resistant to several conventional anti-cancer drugs, NoA may offer a new scaffold for the development of drugs that have the potential to target tumor cells independent of their p53 status. As no analogous type of compound was previously described in the nature, NoA establishes a novel class of bioactive secondary metabolites. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1