Počet záznamů: 1
The First Extracellular Linker Is Important for Several Aspects of the Gating Mechanism of Human TRPA1 Channel
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SYSNO ASEP 0473936 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The First Extracellular Linker Is Important for Several Aspects of the Gating Mechanism of Human TRPA1 Channel Tvůrce(i) Maršáková, Lenka (FGU-C) RID
Barvík, I. (CZ)
Zíma, V. (CZ)
Zímová, Lucie (FGU-C) RID, ORCID
Vlachová, Viktorie (FGU-C) RID, ORCID, SAIČíslo článku 16 Zdroj.dok. Frontiers in Molecular Neuroscience. - : Frontiers Media - ISSN 1662-5099
Roč. 10, Jan 31 (2017)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova TRP channel ; S1-S2 linker ; allyl isothiocynate ; sensor module Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA15-15839S GA ČR - Grantová agentura ČR GBP304/12/G069 GA ČR - Grantová agentura ČR EE2.3.30.0025 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000392971600002 EID SCOPUS 85014040711 DOI 10.3389/fnmol.2017.00016 Anotace Transient receptor potential ankyrin 1 (TRPA1) is an excitatory ion channel involved in pain, inflammation and itching. This channel gates in response to many irritant and proalgesic agents, and can be modulated by calcium and depolarizing voltage. While the closed-state structure of TRPA1 has been recently resolved, also having its open state is essential for understanding how this channel works. Here we use molecular dynamics simulations combined with electrophysiological measurements and systematic mutagenesis to predict and explore the conformational changes coupled to the expansion of the presumptive channel's lower gate. We show that, upon opening, the upper part of the sensor module approaches the pore domain of an adjacent subunit and the conformational dynamics of the first extracellular flexible loop may govern the voltage-dependence of multimodal gating, thereby serving to stabilize the open state of the channel. These results are generally important in understanding the structure and function of TRPA1 and offer new insights into the gating mechanism of TRPA1 and related channels. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1