Počet záznamů: 1
Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide
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SYSNO ASEP 0469671 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide Tvůrce(i) Žárská, Monika (UMG-J)
Novotný, Filip (UMG-J)
Havel, Filip (UMG-J)
Šrámek, Michal (UMG-J)
Babelova, A. (SK)
Benada, Oldřich (MBU-M) ORCID, RID
Novotný, M. (CZ)
Saran, H. (CZ)
Kuca, K. (CZ)
Musílek, K. (CZ)
Hvězdová, Zuzana (UMCH-V)
Dzijak, Rastislav (UMG-J) RID
Vančurová, Markéta (UMG-J)
Krejčíková, Kateřina (UMG-J)
Gabajová, Blanka (UMG-J)
Hanzlíková, Hana (UMG-J) RID
Kyjacová, Lenka (UMG-J)
Bártek, Jiří (UMG-J) RID
Proška, Jan (UMG-J)
Hodný, Zdeněk (UMG-J) RIDCelkový počet autorů 20 Zdroj.dok. Bioconjugate Chemistry. - : American Chemical Society - ISSN 1043-1802
Roč. 27, č. 10 (2016), s. 2558-2574Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova mesenchymal stem-cells ; mammalian-cells ; cancer-cells ; photothermal therapy ; mediated endocytosis ; nonphagocytic cells ; optical-properties ; light-scattering ; senescent cells ; dna-damage Vědní obor RIV EB - Genetika a molekulární biologie Vědní obor RIV – spolupráce Mikrobiologický ústav - Mikrobiologie, virologie
Ústav makromolekulární chemie - Makromolekulární chemieCEP GA16-13967S GA ČR - Grantová agentura ČR LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 ; UMCH-V - RVO:61389013 UT WOS 000385992000039 EID SCOPUS 84992397142 DOI 10.1021/acs.bioconjchem.6b00491 Anotace Cationic colloidal gold nanorods (GNRs) have a great potential as a theranostic tool for diverse medical applications. GNRs' properties such as cellular internalization and stability are determined by physicochemical characteristics of their surface coating. GNRs modified by (16-mercaptohexadecyl)trimethylammonium bromide (MTAB), (MTAB)GNRs, show excellent cellular uptake. Despite their promise for biomedicine, however, relatively little is known about the cellular pathways that facilitate the uptake of GNRs, their subcellular fate and intracellular persistence. Here we studied the mechanism of cellular internalization and long-term fate of GNRs coated with MTAB, for which the synthesis was optimized to give higher yield, in various human cell types including normal diploid versus cancerous, and dividing versus nondividing (senescent) cells. The process of (MTAB)GNRs internalization into their final destination in lysosomes proceeds in two steps: (1) fast passive adhesion to cell membrane mediated by sulfated proteoglycans occurring within minutes and (2) slower active transmembrane and intracellular transport of individual nanorods via clathrin-mediated endocytosis and of aggregated nanorods via macropinocytosis. The expression of sulfated proteoglycans was the major factor determining the extent of uptake by the respective cell types. Upon uptake into proliferating cells, (MTAB)GNRs were diluted equally and relatively rapidly into daughter cells; however, in nondividing/senescent cells the loss of (MTAB)GNRs was gradual and very modest, attributable mainly to exocytosis. Exocytosed (MTAB)GNRs can again be internalized. These findings broaden our knowledge about cellular uptake of gold nanorods, a crucial prerequisite for future successful engineering of nanoparticles for biomedical applications such as photothermal cancer therapy or elimination of senescent cells as part of the emerging rejuvenation approach. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2017
Počet záznamů: 1