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Proteins and their modifications in a medieval mummy
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SYSNO ASEP 0465067 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proteins and their modifications in a medieval mummy Tvůrce(i) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Sedláková, Pavla (FGU-C) RID
Pataridis, Statis (FGU-C)
Bortolotti, F. (IT)
Gottardo, R. (IT)Zdroj.dok. Protein Science. - : Wiley - ISSN 0961-8368
Roč. 25, č. 11 (2016), s. 2037-2044Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova mummy ; collagen ; protein modification ; deamidation ; carbamylation ; carboxymethylation Vědní obor RIV CB - Analytická chemie, separace CEP GA15-01948S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000387151900012 EID SCOPUS 84992388928 DOI 10.1002/pro.3024 Anotace Proteins and their modifications of the natural mummy of Cangrande della Scala (Prince of Verona, Northern Italy, 12911329) were studied. The nano-LC-Q-TOF analysis of samples of rib bone and muscle from the mummy showed the presence of different proteins including Types I, III, IV, V, and XI collagen, hemoglobin (subunits alpha and beta), ferritin, biglycan, vitronectin, prothrombin, and osteocalcin. The structure of Type I and Type III collagen was deeply studied to evaluate the occurrence of modifications in comparison with Type I and Type III collagen coming from tissues of recently died people. This analysis showed high percentage of asparaginyl and glutaminyl deamidation, carbamylation and carboxymethylation of lysine, as well as oxidation and dioxidation of methionine. The most common reaction during the natural mummification process was oxidationthe majority of lysine and proline of collagen Type I was hydroxylated whereas methionine was oxidated (oxidated or dioxidated). To the best of our knowledge, this is the first study which reports the protein profile of a natural mummified human tissue and the first one which describes the carbamylation and carboxymethylation of lysine in mummified tissues. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
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