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Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei
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SYSNO ASEP 0461180 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei Tvůrce(i) Alkhaldi, A.A.M. (GB)
Martínek, Jan (BC-A)
Panicucci, Brian (BC-A)
Dardonville, C. (ES)
Zíková, Alena (BC-A) RID, ORCID
de Koning, H.P. (GB)Zdroj.dok. International Journal for Parasitology-Drugs and Drug Resistance . - : Elsevier - ISSN 2211-3207
Roč. 6, č. 1 (2016), s. 23-34Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Trypanosoma brucei ; mitochondrion ; FoF1 ATPase ; succinate dehydrogenase ; phosphonium salt ; SDH complex Vědní obor RIV EB - Genetika a molekulární biologie CEP LL1205 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 UT WOS 000372717200003 EID SCOPUS 84953438596 DOI 10.1016/j.ijpddr.2015.12.002 Anotace Lipophilic bisphosphonium salts are among the most promising antiprotozoal leads currently under investigation. As part of their preclinical evaluation we here report on their mode of action against African trypanosomes, the etiological agents of sleeping sickness. The bisphosphonium compounds CD38 and AHI-9 exhibited rapid inhibition of Trypanosoma brucei growth, apparently the result of cell cycle arrest that blocked the replication of mitochondrial DNA, contained in the kinetoplast, thereby preventing the initiation of S-phase. Incubation with either compound led to a rapid reduction in mitochondrial membrane potential, and ATP levels decreased by approximately 50% within 1 h. Between 4 and 8 h, cellular calcium levels increased, consistent with release from the depolarized mitochondria. Within the mitochondria, the Succinate Dehydrogenase complex (SDH) was investigated as a target for bisphosphonium salts, but while its subunit 1 (SDH1) was present at low levels in the bloodstream form trypanosomes, the assembled complex was hardly detectable. RNAi knockdown of the SDH1 subunit produced no growth phenotype, either in bloodstream or in the procyclic (insect) forms and we conclude that in trypanosomes SDH is not the target for bisphosphonium salts. Instead, the compounds inhibited ATP production in intact mitochondria, as well as the purified F-1 ATPase, to a level that was similar to 1 mM azide. Co-incubation with azide and bisphosphonium compounds did not inhibit ATPase activity more than either product alone. The results show that, in T. brucei, bisphosphonium compounds do not principally act on succinate dehydrogenase but on the mitochondrial FoF1 ATPase. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
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