Počet záznamů: 1
The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in streptozotocin-induced diabetic mice.\n\n\n
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SYSNO ASEP 0459814 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in streptozotocin-induced diabetic mice.
Tvůrce(i) Valášková, Eliška (BTO-N)
Žatecká, Eva (BTO-N)
Dorosh, Andriy (BTO-N) RID
Pavlínková, Gabriela (BTO-N) RID, ORCID
Bohuslavová, Romana (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Margaryan, Hasmik (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 10 Zdroj.dok. Book of abstract XXIInd Symposium of immunology and biology of Reproduction with international participation. - Vestec u Prahy : Biotechnologický ústav, 2016 / Pěknicová Jana ; Kubátová Alena ; Elzeinová Fatima
S. 42-43Poč.str. 2 s. Akce XXIInd Symposium of Immunology and Biology of Reproduction with international participation Datum konání 26.05.2016 - 28.05.2016 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova diabetes mellitus ; testicular genes analysis ; streptozotocin ; annexin V ; sperm parameters Vědní obor RIV EB - Genetika a molekulární biologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy AZV 15-30880A GA MZd - Ministerstvo zdravotnictví Institucionální podpora BTO-N - RVO:86652036 Anotace According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. Causes of this condition could be genetic background, environmental factors and various diseases, including diabetes mellitus (DM). However, the impact of DM on male fertility is not fully understood. The aim of this study was to investigate the effects of DM on reproduction and expression of selected testicular genes using FVB inbred mouse strain, suitable for inducing DM and subsequent reproductive parameters analysis. This mouse strain is characterized by vigorous reproductive performance and consistently large litters.
DM (type 1) was artificially induced in the age of 6 weeks by chemical substance streptozotocin, which causes destruction of pancreatic β cells. Mice with blood sugar levels higher than 13.9 mmol/L were considered as diabetic. These mice were exposed to diabetic condition for 6 weeks and then subjected to analysis.
Our results have shown that diabetic condition had an impact on body weight, weight of reproductive organs as well as kidneys and livers. We also evaluated sperm quality by analysing several sperm parameters (morphology, viability, concentration, apoptotic status). We observed decreased concentration and viability of diabetic sperm compared to control. We also noticed increased staining with apoptotic marker annexin V. Moreover, we evaluated the qualitative and quantitative changes of sperm nuclear proteins - protamines. In diabetic animals, we observed higher number of sperm with insufficient protamination. Nevertheless, protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was not altered in sperm of diabetic animals compared to controls. Regarding the testicular tissue, we observed impaired morphology of seminiferous tubules and increased number of apoptotic cells in testes of diabetic animals. We also analysed expression of several important testicular genes (Vegfa, Sycp1, Sycp3, Ccna1, Meig1, Prm1, Prm2, Tnp1, Tnp2, Grth, Gja1, Wt1, Sox8, p21, p53, Bcl-2, Ar, Fshr, Star, p450scc), which are responsible for proper function of spermatocytes and spermatids.
Our findings indicate that DM affects sperm quality in terms of alterations in the morphology of testicular tissue. The metabolic disorders also caused changes in the expression of genes important during meiotic phase of spermatogenesis (Sycp1 and Sycp3) and genes important during spermiogenesis (Tnp1, Tnp1, Prm1 and Prm2). Changes in gene expression could further contribute to impaired sperm quality of diabetic animals.
Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1