Počet záznamů: 1
Hybrid Sterility Locus on Chromosome X Controls Meiotic Recombination Rate in Mouse
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SYSNO ASEP 0459797 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hybrid Sterility Locus on Chromosome X Controls Meiotic Recombination Rate in Mouse Tvůrce(i) Balcová, Mária (UMG-J)
Faltusová, Barbora (UMG-J)
Gergelits, Václav (UMG-J)
Bhattacharyya, Tanmoy (UMG-J)
Mihola, Ondřej (UMG-J) RID, ORCID
Trachtulec, Zdeněk (UMG-J) RID, ORCID
Knopf, Corinna (UMG-J)
Fotopulosová, Vladana (UMG-J)
Chvátalová, Irena (UMG-J)
Gregorová, Soňa (UMG-J)
Forejt, Jiří (UMG-J) RID, ORCIDZdroj.dok. PLoS Genetics. - : Public Library of Science - ISSN 1553-7404
Roč. 12, č. 4 (2016), e1005906-e1005906Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Histochine H3 methyltransferase ; Crossing-Over ; Complex Traits Vědní obor RIV EB - Genetika a molekulární biologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA13-08078S GA ČR - Grantová agentura ČR GA14-20728S GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000375231900005 DOI 10.1371/journal.pgen.1005906 Anotace Meiotic recombination safeguards proper segregation of homologous chromosomes into gametes, affects genetic variation within species, and contributes to meiotic chromosome recognition, pairing and synapsis. The Prdm9 gene has a dual role, it controls meiotic recombination by determining the genomic position of crossover hotspots and, in infertile hybrids of house mouse subspecies Mus m. musculus (Mmm) and Mus m. domesticus (Mmd), it further functions as the major hybrid sterility gene. In the latter role Prdm9 inter-acts with the hybrid sterility X 2 (Hstx2) genomic locus on Chromosome X (Chr X) by a still unknown mechanism. Here we investigated the meiotic recombination rate at the genomewide level and its possible relation to hybrid sterility. Using immunofluorescence microscopy we quantified the foci of MLH1 DNA mismatch repair protein, the cytological counterparts of reciprocal crossovers, in a panel of inter-subspecific chromosome substitution strains. Two autosomes, Chr 7 and Chr 11, significantly modified the meiotic recombination rate, yet the strongest modifier, designated meiotic recombination 1, Meir1, emerged in the 4.7 Mb Hstx2 genomic locus on Chr X. The male-limited transgressive effect of Meir1 on recombination rate parallels the male-limited transgressive role of Hstx2 in hybrid male sterility. Thus, both genetic factors, the Prdm9 gene and the Hstx2/Meir1 genomic locus, indicate a link between meiotic recombination and hybrid sterility. A strong female-specific modifier of meiotic recombination rate with the effect opposite to Meir1 was localized on Chr X, distally to Meir1. Mapping Meir1 to a narrow candidate interval on Chr X is an important first step towards positional cloning of the respective gene(s) responsible for variation in the global recombination rate between closely related mouse subspecies. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2017
Počet záznamů: 1