Počet záznamů: 1
Possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm
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SYSNO ASEP 0454481 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm Tvůrce(i) Margaryan, Hasmik (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Čapková, Jana (BTO-N)
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCID
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 7 Zdroj.dok. Abstracts of the 14th International Symposium for Immunology of Reproduction “Progress in Reproductive Immunology”. - Hoboken : American Journal of Reproductive Immunology, 2015 / Mor Gil - ISSN 1046-7408
S. 40Poč.str. 1 s. Akce 14th International Symposium for Immunology of Reproduction "progress in Reproductive Immunology" Datum konání 22.05.2015-24.05.2015 Místo konání Varna Země BG - Bulharsko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova monoclonal antibodies ; spermatozoa ; GAPDHS ; immunolabeling ; Transmission electron microscopy ; in vitro sperm/zona pellucida binding assay Vědní obor RIV CE - Biochemie CEP GAP503/12/1834 GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) Anotace Sperm proteins are important in fertilization. Some of them are involved in the binding of sperm to the egg.We characterized the sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory. In immunoblotting test, MoAb Hs-8 labelled a protein of 45 kDa in the extract of sperm. Sequence analysis identified protein Hs-8 as GAPDHS. For this reason, commercial mouse anti-GAPDHS MoAb was applied in control tests. Both antibodies showed similar staining patterns in immunofluorescence tests, in electron microscopy and in immunoblot analysis. Moreover, both Hs-8 and anti-GAPDHS antibodies blocked sperm/zona pellucida binding. GAPDHS is a sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility; its role in the sperm head is unknown. In this study, we confirmed the potential role of GAPDHS as a binding protein that is involved in the sperm/oocyte binding. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2016
Počet záznamů: 1