Počet záznamů: 1
Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes
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SYSNO ASEP 0453397 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes Tvůrce(i) Lieskovská, Jaroslava (BC-A) RID
Páleníková, Jana (BC-A)
Langhansová, Helena (BC-A) RID
Chagas, A. C. (US)
Calvo, E. (US)
Kotsyfakis, Michalis (BC-A) RID, ORCID
Kopecký, Jan (BC-A) RIDZdroj.dok. Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 8, MAY 15 2015 (2015), s. 275Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Dendritic cells ; Borrelia burgdorferi ; Tick cystatin ; Signalling Vědní obor RIV EC - Imunologie CEP GAP302/12/2208 GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000354848900001 DOI 10.1186/s13071-015-0887-1 Anotace We determined the effect of cystatins on the production of chemokines in Borrelia-infected bone-marrow derived DC. The production of MIP-1 alpha was severely suppressed by both cystatins, while IP-10 was selectively inhibited only by Sialo L2. As TLR-2 is a major receptor activated by Borrelia spirochetes, we tested whether cystatins influence signalling pathways activated by TLR-2 ligand, lipoteichoic acid (LTA). Sialo L2 and weakly Sialo L attenuated the extracellular matrix-regulated kinase (Erk1/2) pathway. The activation of phosphatidylinositol-3 kinase (PI3K)/Akt pathway and nuclear factor-kappa B (NF-kappa B) was decreased only by Sialo L2. In response to Borrelia burgdorferi, the activation of Erk1/2 was impaired by Sialo L2. Production of IFN-beta was analysed in plasmacytoid DC exposed to Borrelia, TLR-7, and TLR-9 ligands. Sialo L, in contrast to Sialo L2, decreased the production of IFN-beta in pDC and also impaired the maturation of these cells. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
Počet záznamů: 1