Počet záznamů: 1
A frame-supported ultrathin electrospun polymer membrane for transplantation of retinal pigment epithelial cells
- 1.
SYSNO ASEP 0446658 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A frame-supported ultrathin electrospun polymer membrane for transplantation of retinal pigment epithelial cells Tvůrce(i) Popelka, Štěpán (UMCH-V) RID
Studenovská, Hana (UMCH-V) RID, ORCID
Abelová, Lucie (UMCH-V) RID
Ardan, Taras (UZFG-Y) RID, ORCID
Studený, P. (CZ)
Straňák, Z. (CZ)
Klíma, Jiří (UZFG-Y) RID, ORCID
Dvořánková, B. (CZ)
Kotek, Jiří (UMCH-V) RID
Hodan, Jiří (UMCH-V)
Rypáček, František (UMCH-V) RIDZdroj.dok. Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 10, č. 4 (2015), 045022_1-045022_15Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova RPE transplantation ; RPE carrier ; porcine RPE Vědní obor RIV CD - Makromolekulární chemie Vědní obor RIV – spolupráce Ústav živočišné fyziologie a genetiky - Makromolekulární chemie CEP GAP108/12/1629 GA ČR - Grantová agentura ČR EE2.3.30.0029 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMCH-V - RVO:61389013 ; UZFG-Y - RVO:67985904 UT WOS 000360215600023 EID SCOPUS 84982810079 DOI 10.1088/1748-6041/10/4/045022 Anotace We report on the design and fabrication of a frame-supported nanofibrous membrane for the transplantation of retinal pigment epithelial (RPE) cells, which is a promising therapeutic option for the treatment of degenerative retinal disorders. The membranous cell carrier prepared from 640 nm-thick poly(DL-lactide) fibres uniquely combines high porosity, large pore size and low thickness, to maximize the nutrient supply to the transplanted cells in the subretinal space and thus to enhance the therapeutic effect of the transplantation. The carrier was prepared by electrospinning, which made it easy to embed a 95 μm-thick circular supporting frame 2 mm in diameter. Implantations into enucleated porcine eyes showed that the frame enabled the ultrathin membrane to be handled without irreversible folding, and allowed the membrane to regain its flat shape when inserted into the subretinal space. We further demonstrated that the minimum membrane thickness compatible with the surgical procedure and instrumentation employed here was as low as 4 μm. Primary porcine RPE cells cultivated on the membranes formed a confluent monolayer, expressed RPE-specific differentiation markers and showed transepithelial resistance close to that of the native RPE. Most importantly, the majority of the RPE cells transplanted into the subretinal space remained viable. The ultrathin, highly porous, and surgically convenient cell carrier presented here has the potential to improve the integration and the functionality of transplanted RPE cells. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2016
Počet záznamů: 1