Počet záznamů: 1
Cytotoxic Lipopeptide Muscotoxin A, Isolated from Soil Cyanobacterium Desmonostoc muscorum, Permeabilizes Phospholipid Membranes by Reducing Their Fluidity
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SYSNO ASEP 0445230 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytotoxic Lipopeptide Muscotoxin A, Isolated from Soil Cyanobacterium Desmonostoc muscorum, Permeabilizes Phospholipid Membranes by Reducing Their Fluidity Tvůrce(i) Tomek, P. (CZ)
Hrouzek, Pavel (MBU-M) ORCID
Kuzma, Marek (MBU-M) ORCID, RID
Sýkora, Jan (UFCH-W) RID
Fišer, R. (CZ)
Černý, J. (CZ)
Novák, Petr (MBU-M) RID, ORCID
Bártová, Simona (MBU-M)
Šimek, Petr (BC-A) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Kavan, Daniel (MBU-M) RID, ORCID
Kopecký, Jiří (MBU-M) ORCIDZdroj.dok. Chemical Research in Toxicology - ISSN 0893-228X
Roč. 28, č. 2 (2015), s. 216-224Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova BLUE-GREEN-ALGA ; CYCLIC-PEPTIDES ; LYNGBYA SP Vědní obor RIV EE - Mikrobiologie, virologie Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Mikrobiologie, virologie
Biologické centrum (od r. 2006) - Mikrobiologie, virologieCEP GPP503/12/P614 GA ČR - Grantová agentura ČR LH11129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 ; UFCH-W - RVO:61388955 ; BC-A - RVO:60077344 UT WOS 000349656100008 EID SCOPUS 84945361419 DOI 10.1021/tx500382b Anotace There is mounting evidence that cyanobacterial lipopeptides can kill mammalian cells, presenting a hazard to human health. Unfortunately, their mechanism of toxicity is poorly understood. We have isolated new cyclic undeca-lipopeptides muscotoxin A and B containing unique lipophilic residue 3-amino-2,5-dihydroxydecanoic acid (5-OH Ahdoa). Muscotoxin B was not used for biological studies due to its poor yield. Muscotoxin A was cytotoxic to YAC-1, Sp/2, and HeLa cancer cell lines (LC50 ranged from 9.9 to 13.2 mu M after 24 h of exposure), causing membrane damage and influx of calcium ions. Subsequently, we studied this lytic mechanism using synthetic liposomes with encapsulated fluorescent probes. Muscotoxin A permeabilized liposomes composed exclusively of phospholipids, demonstrating that no proteins or carbohydrates present in biomembranes are essential for its activity. Paradoxically, the permeabilization activity of muscotoxin A was mediated by a significant reduction in membrane surface fluidity (stiffening), the opposite of that caused by synthetic detergents and cytolytic lipopeptide puwainaphycin F. At 25 degrees C, muscotoxin A disrupted liposomes with and without cholesterol/sphingomyelin; however, at 37 degrees C, it was selective against liposomes with cholesterol/sphingomyelin. It appears that both membrane fluidity and organization can affect the lytic activity of muscotoxin A. Our findings strengthen the evidence that cyanobacterial lipopeptides specifically disrupt mammalian cell membranes and bring new insights into the mechanism of this effect. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2016
Počet záznamů: 1