Počet záznamů: 1
Clustered DNA damage on subcellular level: effect of scavengers
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SYSNO ASEP 0436873 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Clustered DNA damage on subcellular level: effect of scavengers Tvůrce(i) Pachnerová Brabcová, Kateřina (UJF-V) RID, ORCID, SAI
Sihver, L. (SE)
Yasuda, N. (JP)
Matuo, Y. (JP)
Štěpán, Václav (UJF-V) RID, ORCID, SAI
Davídková, Marie (UJF-V) RID, ORCID, SAICelkový počet autorů 6 Zdroj.dok. Radiation and Environmental Biophysics - ISSN 0301-634X
Roč. 53, č. 4 (2014), s. 705-712Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova clustered damage ; indirect effects ; haevy ion ; plasmid in liquid water ; scavenger Vědní obor RIV BO - Biofyzika CEP LD12008 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UJF-V - RVO:61389005 UT WOS 000344177800008 EID SCOPUS 84904272899 DOI https://doi.org/10.1007/s00411-014-0557-2 Anotace Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers. Pracoviště Ústav jaderné fyziky Kontakt Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Rok sběru 2015
Počet záznamů: 1