Počet záznamů: 1  

Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes

  1. 1.
    SYSNO ASEP0436793
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEpigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes
    Tvůrce(i) Vlková, Veronika (UMG-J)
    Štěpánek, Ivan (UMG-J) RID
    Hrušková, Veronika (UMG-J)
    Šenigl, Filip (UMG-J) RID
    Mayerová, Veronika (UMG-J)
    Šrámek, Martin (UMG-J)
    Šímová, Jana (UMG-J) RID
    Bieblová, Jana (UMG-J)
    Indrová, Marie (UMG-J) RID
    Hejhal, Tomáš (UMG-J)
    Dérian, N. (FR)
    Klatzmann, D. (FR)
    Six, A. (FR)
    Reiniš, Milan (UMG-J) RID
    Zdroj.dok.OncoTarget. - : Impact Journals LLC - ISSN 1949-2553
    Roč. 5, č. 16 (2014), s. 6923-35
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaIFNγ signalling pathway ; DNA demethylation ; tumour
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPGAP301/10/2174 GA ČR - Grantová agentura ČR
    NT14461 GA MZd - Ministerstvo zdravotnictví
    Institucionální podporaUMG-J - RVO:68378050
    UT WOS000347920100033
    AnotaceDownregulation of MHC class I expression on tumour cells, a common mechanism by which tumour cells can escape from specific immune responses, can be associated with coordinated silencing of antigen-presenting machinery genes. The expression of these genes can be restored by IFNγ. In this study we documented association of DNA demethylation of selected antigen-presenting machinery genes located in the MHC genomic locus (TAP-1, TAP-2, LMP-2, LMP-7) upon IFNγ treatment with MHC class I upregulation on tumour cells in several MHC class I-deficient murine tumour cell lines (TC-1/A9, TRAMP-C2, MK16 and MC15). Our data also documented higher methylation levels in these genes in TC-1/A9 cells, as compared to their parental MHC class I-positive TC-1 cells. IFNγ-mediated DNA demethylation was relatively fast in comparison with demethylation induced by DNA methyltransferase inhibitor 5-azacytidine, and associated with increased histone H3 acetylation in the promoter regions of APM genes. Comparative transcriptome analysis in distinct MHC class I-deficient cell lines upon their treatment with either IFNγ or epigenetic agents revealed that a set of genes, significantly enriched for the antigen presentation pathway, was regulated in the same manner. Our data demonstrate that IFNγ acts as an epigenetic modifier when upregulating the expression of antigen-presenting machinery genes.
    PracovištěÚstav molekulární genetiky
    KontaktNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Rok sběru2015
Počet záznamů: 1  

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